The chick embryo – past, present and future as a model system in developmental biology 1011 PART I: The chick as a classical system for the study of development L. Wolpert (UK) Much more from the chicken's egg than breakfast – a wonderful model system 1015 C. Tickle (UK) The contribution of chicken embryology to the understanding of vertebrate limb development 1019 L. Wittler, M. Kessel (German...

1. The changes in the net amounts of retinol, retinyl esters and retinal in both the developing chick embryo and the newly hatched chick were investigated. The embryo requires about 68nmol of the vitamin for its growth, whereas the baby chick requires about 108nmol during the first 7 days after hatching. 2. Retinal was present in the egg in fairly high concentrations at the beginning of the inc...

Ott (1, 2) has shown that desoxypyridoxine (2,4-dimethyl-3-hydroxy5hydroxymethylpyridine) and methoxypyridoxine (2-methyl-3-hydroxy4-methoxymethyl-5-hydroxymethylpyridine), analogues of pyridoxine, act as pyridoxine antagonists in the chick. Because of our interest in the effects of metabolic antagonists on the development of the chick embryo (3), and on the behavior of mouse tumors growing on ...

In ovo studies on the effect of 1,3,5, ppm Bioneem (0.03%) formulation on Biochemical aspect of chick embryo revealed that there was dose dependent total protein reduction in 96 hrs old embryo (treated at 24 hrs) as compared to the control. Also there was reduction in total protein concentration Liver, Brain and Heart of 15 day old chick embryo (treated with Bioneem at 96 hrs. stage) as compare...

Fertilized chicken eggs are suggested as an alternative to mammalian models. The chorioallantoic membrane (CAM) of the chick embryo is widely used for examination of angiogenesis, xenotransplants and for virus production. Unfortunately, it is mostly not taken into account, that the chick embryo's ability to experience pain starts to develop at day 7 of breeding. In our view, this model is only ...

A comparative study on tumorigenicity was carried out in which two Epstein-Barr virus (EBV) genome-positive epithelial cell lines (A2L/AH and NPC-KT) were tested in nude mice xenograft system and chick embryo system. When two cell lines were inoculated subcutaneously in nude mice and on chorioallantonic membrane of the chick embryo, respectively, tumors developed in both systems. Histologically...

Antibodies to pure lysyl hydroxylase from whole chick embryos were prepared in rabbits and used for immunological characterization of this enzyme of collagen biosynthesis. In double immunodiffusion a single precipitation line was seen between the antiserum and crude or pure chick-embryo lysyl hydroxylase. The antiserum effectively inhibited chick-embryo lysyl hydroxylase activity, whether measu...

An assay capable of recovering individual viable rodent cells localized in various organs of the chick embryo is described. This assay is based on the differential sensitivity of chick and rodent cells to the cytotoxic drug ouabain. Utilizing this assay, the potential of the chick embryo as a model system for studying metastasis was examined. Several cell lines were characterized in three ways:...

1. Radioisotope incorporation studies of normal and lathyritic chick embryo bone collagen do not demonstrate any interference by lathyrism with collagen synthesis or fibril formation. 2. The results indicate that a portion of the extractable collagen from lathyritic chick embryo bone represents newly synthesized protein. Evidence from a double labeling experiment and from analysis of isotope fl...

The complete primary structure of a new extracellular protein associated with elastic fiber microfibrils was determined by recombinant DNA techniques. Antiserum to insoluble bovine ocular zonule protein was used to screen a lambda gt11 cDNA expression library constructed from whole chick embryo poly(A)+ RNA. The cDNAs encoding immunoreactive fusion polypeptides were then used to rescreen the li...