IN T RO DU C T IO N To maximize return on investment, the chemical industry is challenged to bring new products to the market while improving productivity. In many cases, developing a new product or a new process with only a small percentage increase in the yield can result in significant increases in earnings for the company. To develop new chemicals and improve organic synthesis efficiency, t...

The chemical synthesis of backbone deuterium labelled [r(CGCGAAU*U*CGCG)]2 (U* = [5'-2H]U) is described. An efficient purification procedure was developed using a polymeric reverse phase (PRP) HPLC column at 60 degrees C. This procedure provided pure RNA dodecamer in the multi-milligram quantities (39% overall yield) necessary for dynamics studies using solid-state deuterium NMR. The purificati...

* Correspondence: [email protected]

We wish to describe certain simplifications in the technique previously described (13, 14) for the purification of prothrombin and thrombin. With new adaptations it is possible to process large quantities of plasma and at the same time to obtain products 2 to 5 times as potent as those previously reported from this laboratory. A brief survey of the chemical properties of these newly obtained pr...

BACKGROUND AND OBJECTIVES Streptokinase is used clinically as an intravenous thrombolytic agent for the treatment of acute myocardial infarction and is commonly prepared from cultures of Streptococcus equisimilis strain H46A. The objective of the present study was the production of streptokinase from strain H46A and purification by chemical reduction method. MATERIALS AND METHODS The rate of ...