ASSAY CURVES, USING A DISK DIFFUSION METHOD FOR THE ANTIBIOTICS GENTAMICIN AND CEFAZOLIN, WERE PREPARED WITH: saline, saline plus 10% serum, and ascitic, synovial, cerebrospinal, and pleural fluids. The curves were compared with a standard curve prepared with pooled human serum. The pH, total protein, glucose, blood urea nitrogen, sodium, potassium, calcium, phosphorus, chloride, CO(2) content,...

The identification of disease markers in tissues and body fluids requires an extensive and thorough analysis of its protein constituents. In our efforts to identify biomarkers for affective and neurological disorders we are pursuing several different strategies. On one hand we are using animal models that represent defined phenotypes characteristic for the respective disorder in humans. In addi...

Using reversed-phase "high-performance" liquid chromatography and hydrophilic ion-pairing reagents, we studied the chromatographic profiles of corticotropin immunoreactivities in human plasma, cerebrospinal fluid, and urine. The chromatographic system we applied provides excellent separation of peptide molecules, with reproducibility (CV) of retention times better than 0.5%, and makes feasible ...

The nasopharynx of known meningococcal carriers without signs of acute meningococcal disease as well as cerebrospinal fluid from patients with acute meningococcal disease were cultured on Thayer-Martin agar. Pili were observed in negatively stained preparations of over 80% cells from all primary cultures of both nasopharnyx and cerebrospinal fluids. Although pili were abundant on cells from al...

Reports of fluorescent treponemal antibody (FTA) testing of cerebrospinal fluids have appeared in the literature from time to time (Harris, Bossak, Deacon, and Bunch, 1960; Vaisman and Hamelin, 1961; Niel and Fribourg-Blanc, 1964). It has become accepted practice in our laboratory to perform an FTA test, without absorption with Reiter treponemes, on undiluted cerebrospinal fluid (CSF). As no es...

Cerebrospinal fluid was obtained by lumbar puncture. Only fluids containing fewer than 100 red blood cells/ c.mm. were studied. The cerebrospinal fluid protein was concentrated by the technique of Grant (1957), in most cases to between 500 and 1,000 mg./100 ml. Cerebrospinal fluids with protein concentrations already in this range were not further concentrated. The details of the immunochemical...

Normal responses of measles specific immunoglobulins M and G (IgM and IgG) were defined in 10 children with measles. Abnormal responses of measles IgM and IgG were found in both sera and cerebrospinal fluids from three cases of subacute sclerosing panencephalitis. In two patients the serum titres of measles IgM and IgG were abnormally high. The measles IgM was present during prolonged illnesses...

Over the past decade, significant improvements in immunochemical and electrophoretic techniques have enabled collection of heretofore unavailable data on proteins in biological fluids, greatly increasing our understanding of protein physiology in the various body compartments and providing the foundation for clinical use of protein analysis in body fluids. The most striking advance has been in ...