The heat inactivation of Lactococcus lactis was studied by determination of cell counts, and by FT-IR spectroscopy recording the average structure of cell proteins. Cell counts were measured after incubation milk buffer or milk buffer with 1. 5 M sucrose, and FT-IR spectra were recorded in (2)H(2)O or (2)H(2)O with 1. 5 M sucrose in the range of 6-75 degrees Celsius. Sucrose protected L. lactis...

Cell pastes and supernatant Escherichia coli samples, taken from an industrial bioprocess overproducing recombinant a2 IFN were analysed using pyrolysis mass spectrometry (PyMS) and diffuse reflectance-absorbance Fourier transform infrared spectroscopy (FT-IR). PyMS and FT-IR are physico-chemical methods which measure predominantly the bond strengths of molecules and the vibrations of bonds wit...

the effect of acetate ion introduced to aqueous solutions was investigated for the extraction/separation of ni and co from a dilute sulfate media. the separation of co and ni was improved by introducing acetate to aqueous media. by the use of the slope analysis method,the stoichiometric coefficient of the extractant was found to be 4 for cobalt and 3.5 or 4 for nickel depending on the acetate c...

copper (cu) is an essential trace element involved in normal reproduction but its over-exposure may produce some detrimental effects. the aim of this study was to investigate the effects of purslane on copper sulfate poisoning on liver structures changes. twenty eight wistar rats were randomly allocated to four treatment groups. group i) control, group ii) copper sulfate (200 mg/kg bw were appl...

Fourier transform infrared (FT-IR) spectroscopy and chemometric techniques were used to discriminate five closely related Salmonella enterica serotype Enteritidis phage types, phage type 1 (PT1), PT1b, PT4b, PT6, and PT6a. Intact cells and outer membrane protein (OMP) extracts from bacterial cell membranes were subjected to FT-IR analysis in transmittance mode. Spectra were collected over a wav...

background: all aerobically growing organisms suffer from exposure to oxidative stress, caused by partially reduced forms of molecular oxygen, known as reactive oxygen species (ros). these are highly reactive and capable of damaging cellular constituents such as dna, lipids and proteins. consequently, cells from many different organisms have evolved mechanisms to protect their components agains...

Background: Quercetin has been reported to modulate cell proliferation and apoptosis. The present study aimed at identifying whether treatment of ionizing radiation (IR) combined with quercetin induces apoptosis in HepG2 cells. Materials and Methods: HepG2 cells were plated at an appropriate density according to each experimental scale and irradiated with 1, 5 and 10 Gy gamma-rays from a 60Co s...

Fourier-transform infrared (FT-IR) microspectroscopic mapping can be used to distinguish between different tissue structures, and to increase the image contrast between normal and cancerous regions of a given tissue sample. This study demonstrates the biochemical changes associated with a consistent link between cancerous tissue and various molecular changes in the IR spectra of human pancreati...