β-Cell insulin secretion is dependent on proper mitochondrial function. Various studies have clearly shown that the Nr4a family of orphan nuclear receptors is essential for fuel utilization and mitochondrial function in liver, muscle, and adipose. Previously, we have demonstrated that overexpression of Nr4a1 or Nr4a3 is sufficient to induce proliferation of pancreatic β-cells. In this study, we...

Methylene blue (MB), a tricyclic phenothiazine drug, has been reported to enhance mitochondrial functions including mitochondrial respiration. By comparison, stress associated with abrupt ethanol withdrawal (EW) impedes mitochondrial functions. We investigated whether MB protects mitochondrial respiration and cell survival from EW stress through a key mitochondrial enzyme, cytochrome c oxidase ...

of heart and genuine sympathy which he showed towards his patients' (Munk's Roll, Vol. IV). These qualities were appreciated by the poorest as well as by the highest in the land. He was an excellent teacher of clinical medicine; and students at four hospitals-Charing Cross, the London, University College and Great Ormond Street Hospitals-valued and owed much to his instruction. Early in his car...

Global warming implies the risk of a changing oxygen regime in seas and oceans our planet. The mitochondrial complex nuclear erythrocytes cartilaginous fish, as energy basis blood cells, has repeatedly encountered such climatic fluctuations throughout their evolutionary history. In this regard, features adaptive strategy erythrocyte thornback ray (Raja clavata L.) are interest from ecological p...

AIMS/HYPOTHESIS Studies on beta cell metabolism are often conducted in rodent beta cell lines due to the lack of stable human beta cell lines. Recently, a human cell line, EndoC-βH1, was generated. Here we investigate stimulus-secretion coupling in this cell line, and compare it with that in the rat beta cell line, INS-1 832/13, and human islets. METHODS Cells were exposed to glucose and pyru...

Endogenous and glucose respiration were studied during the life history of Chlorella pyrenoidosa. A generalized picture of the course of respiration during the life cycle is suggested. At the liberation of daughter cells from the wall of the mother cell, or soon after, the respiration rate reaches its lowest level. If the daughter cells are placed in light the respiration rate rapidly increases...

BACKGROUND In this investigation the effects of tricyclic drugs on cellular respiration were studied using the anaplastic astrocytoma cell line IPSB-18 by use of a Clark-type oxygen electrode which measured changes in cellular respiration rate (oxygen consumption), in a dose-response assay. MATERIALS AND METHODS The drugs investigated were clomipramine, norclomipramine, amitriptyline and doxe...

OBJECTIVE Mitochondrial DNA (mtDNA) damage is present in murine and human atherosclerotic plaques. However, whether endogenous levels of mtDNA damage are sufficient to cause mitochondrial dysfunction and whether decreasing mtDNA damage and improving mitochondrial respiration affects plaque burden or composition are unclear. We examined mitochondrial respiration in human atherosclerotic plaques ...

Salicylic acid (SA) inhibited germination of cocklebur (Xanthium pennsylvanicum Wlr), lower seeds, which are completely after-ripened and nondormant. SA also inhibited ethylene production during a pre-germination period of the seeds. Exogenous ethylene overcame the inhibtion of the seed germination by SA. Moreover, SA reduced respiration in pre-germinating whole seeds as well as excised axial...