Disintegration of microbial cells is a necessary first step for the production of intracellular enzymes and organelIes. With increasing use o f intracellular microbial material in industry and medicine, the cell disruption unit operation is gaining in importance. This review examines the state of the art o f the largescale cell disruption technology and disruption methods o f potential commerci...

Microbial cells were disintegrated in a new type of rotary disintegrator with a disc stirrer by a combination of shear force layers, collisions, and rolling of glass beads which were brought into motion by the stirrer. The rate of disintegration at a given dry bed volume of Ballotini beads and a given volume of cell suspension is proportional to the peripheral velocity of the stirrer up to 18 m...

In order to quantitate lymphocyte proliferative responses, we explored the role of cell death in the kinetics of phytohemagglutinin-stimulated cultures. Unless the disintegration time (tDIS) of nonviable lymphocytes in culture is known, the rate of cell death cannot be calculated. To obtain tDIS, we determined the time interval between total and viable cell population decay after various killin...

We show that the existence of disintegration for cylindrical measures follows from a general disintegration theorem for countably additive measures.

Disintegration by hydrodynamic cavitation has a positive effect on the degree and rate of sludge anaerobic digestion. By applying hydrodynamic disintegration the lysis of cells occurs in minutes instead of days. The intracellular and extracellular components are set free and are immediately available for biological degradation which leads to an improvement of the subsequent anaerobic process. H...

Drug product performance testing is an important part of quality-by-design approaches, but this process often lacks the underlying mechanistic understanding of the complex interactions between the disintegration and dissolution processes involved. Whereas a recent draft guideline by the US Food and Drug Administration (FDA) has allowed the replacement of dissolution testing with disintegration ...