Microfluidic technology provides precise, controlled-environment, cost-effective, compact, integrated, and high-throughput microsystems that are promising substitutes for conventional biological laboratory methods. In recent years, microfluidic cell culture devices have been used for applications such as tissue engineering, diagnostics, drug screening, immunology, cancer studies, stem cell prol...

Many human breast cancer cell lines have been in culture for several years, serving as model systems for studying aspects of breast cancer biology. Molecular alterations might occur in these cells during cultivation, and it remains unknown to which extent findings in these cell lines can be related to human disease. Hereby, we describe the establishment of a breast cancer cell line, MW1, from m...

A process comprising two-step fed-batch cultivation has been investigated for the production of ectoines using the halophilic bacterium Halomonas boliviensis DSM 15516(T). The first cultivation was performed under optimal conditions for cell growth and resulted in cell mass concentration of about 41 g l(-1) after 24 h of cultivation. During the second cultivation at higher salt concentration, a...

Rifamycins are broad spectrum and important antibiotics used in the treatment of pathogenic diseases. They are generally produced by the fermentative cultivation of Amycolatopsis mediterranei, using submerged cultivation system. The medium composition greatly influences the antibiotic production. Trace elements are one of the major factors need to be optimized to enhance the production of rifam...

In recent years, circulating progenitors of endothelial cells and smooth muscle cells were identified in the peripheral blood. In our study, we evaluated the utilization of both cell types isolated and differentiated from peripheral porcine blood in terms for their use for tissue engineering purposes. By means of density gradient centrifugation, the monocyte fraction from porcine blood was sepa...

A cell suspension culture of sunflower (Helianthus annuus), a producer of immunologically active polysaccharides, was cultivated in a 5-L stirred tank bioreactor, operated in batch mode. After some changes in the internal bioreactor design a stable growth of Helianthus cells was achieved and the accumulated biomass reached 15.2 g/L (only approximately 5% lower compared to the accumulated biomas...

Citation: Boughattas S (2015) Commentary on: “Detection of Toxoplasma gondii in raw caprine, ovine, buffalo, bovine, and camel milk using cell cultivation, cat bioassay, capture ELISA, and PCR methods in Iran”. Front. Microbiol. 6:215. doi: 10.3389/fmicb.2015.00215 Commentary on: “Detection of Toxoplasma gondii in raw caprine, ovine, buffalo, bovine, and camel milk using cell cultivation, cat b...

Dynamic cultivation of scaffolds loaded with undifferentiated stem cells can lead toward osteogenic differentiation in vivo. The aim of this study was to examine the influence of different in vitro cultivation setups on the integration of cell-matrix constructs after subcutaneous implantation. Human mesenchymal stem cells (hMSC) were inoculated on clinically approved scaffolds. These cell-matri...

introduction: nowadays, spermatogonial stem cells (sscs) cultivation has been used by many researchers as an effective tool for infertility treatments. oxidative conditions can be effective on cell proliferation and differentiation of these cells. so, the aim of this study was to establish oxidative stress model for antioxidant activity of some drugs investigation during sscs in vitro culture. ...