OBJECTIVE To develop a simple rapid procedure for bioreduction of silver nanoparticles (AgNPs) using aqueous leaves extracts of Catharanthus roseus (C. roseus). METHODS Characterization were determined by using UV-Vis spectrophotometry, scanning electron microscopy (SEM), energy dispersive X-ray and X-ray diffraction. RESULTS SEM showed the formation of silver nanoparticles with an average ...

A cDNA encoding a bHLH transcription factor was isolated by the yeast one-hybrid system from a Catharanthus roseus cDNA library using the G-box element of the Strictosidine synthase gene promoter as bait. The corresponding protein (named CrMYC1) was shown to bind specifically to the G-box in yeast. In C. roseus suspension cells CrMYC1 mRNA levels are induced by fungal elicitor and jasmonate sug...

A field experiment was conducted during two successive seasons 2010 and 2011 to determine the influence of rabbit manure rats( 5,10 and 15 m / fed.) and bio-fertilizers. The biofertilizer used was Bacillus sirculans (B.S) (used as seedlings inoculants) and vesicular arbuscular mycorrhizal fungi (VAM) (used as soil inoculants) and their interactions on periwinkle (Catharanthus roseus , L. G. Don...

A comprehensive metabolomic profiling of Catharanthus roseus L. G. Don infected by 10 types of phytoplasmas was carried out using one-dimensional and two-dimensional NMR spectroscopy followed by principal component analysis (PCA), an unsupervised clustering method requiring no knowledge of the data set and used to reduce the dimensionality of multivariate data while preserving most of the varia...

Endophytic microorganisms are recognized as potential source of novel chemical molecules that might be useful in the treatment of infectious diseases. This study was conducted with the aim to isolate and identify endophytic bacteria from medicinal plants recommended for cancer therapy with a potential to produce L-asparaginase. Five different plants were selected to enhance diversity as Cathara...

Feeding deterrents or antifeedant chemicals are those chemicals which inhibit feeding of insect on a treated food material without necessarily killing or repelling them. Plants with ant feeding properties are increasingly being used against phytophagous insect pests for protection of crops. In the present study of growth regulating action of Leaf extract of Catharanthus roseus on development st...

from Suspension-Cultured Catharanthus roseus Cells* Yüko Yamashita and Hiroshi Ashihara Department of Biology, Faculty of Science, Ochanomizu University, 2-1-1, Otsuka, Bunkyo-ku, Tokyo, 112, Japan Z. Naturforsch. 43c, 827-834 (1988); received June 28, 1988 Catharanthus roseus, Suspension Culture, Fructokinase, Glycolysis, Hexokinase Two different hexose-phosphorylating enzymes, hexokinase and ...

For biotransformational studies, Callus of Catharanthus roseus was produced from young shoot tip on Murashige & Skoog medium supplemented with 1.5 mg/litre 2, 4-D and 0.5 mg/litre Kin, solidified by 8 g/litre agar at 25±1°C under complete darkness. The cultures were maintained on the same medium at 25±1 °C in the dark by subculturing after every four weeks. Suspension culture of the plant was p...

A cell suspension culture o f Catharanthus roseus was trea ted during the grow th phase w ith the nucleoside analogue 5-azacytidine, a powerful inducer o f cell d ifferen tia tion , as evidenced w ith animal and hum an cell lines. After induction the cell cu lture was fu rth er incubated in a production m edium in the absence o f the inducer for 10 days. T he extraction o f the freeze dried cel...

Catharanthus roseus is an important medicinal plant known for its pharmacological qualities such as antimicrobial, anticancerous, antifeedant, antisterility, antidiabetic activities. More than 130 bioactive compounds like vinblastine, vindoline and vincristine have been synthesized in this plant. Extensive studies have been carried out for optimization regeneration and transformation protocols....