Catalase-peroxidase (KatG) is an enzyme capable of utilizing both catalase and peroxidase activity to break down hydrogen peroxide. Catalase activity by the enzyme generally dominates, but peroxidatic electron donors have been shown to stimulate catalase activity, likely by rescuing catalase-inactive intermediates resulting from off-pathway electron transfer. To investigate tryptophan 438 as a ...

The in vivo effects of 3-amino-1,2,4-triazole (AT) on the fine structure of microbodies in hepatic cells of male rats has been studied by the peroxidase-staining technique. Within 1 hr of intraperitoneal injection AT abolishes microbody peroxidase-staining, and the return of staining coincides temporally with the known pattern of return of catalase activity following AT inhibition; this is furt...

Caulobacter crescentus is an obligate aerobe which is exposed to high concentrations of photosynthetic oxygen and low levels of nutrients in its aquatic environment. Physiological studies of oxidative and starvation stresses in C. crescentus were undertaken through a study of lacZ fusion and null mutant strains constructed from the cloned 5' end of katG, encoding a catalase-peroxidase. The katG...

Stable films of dimyristoylphosphatidylcholine and M. tuberculosis catalase-peroxidase (KatG), several peroxidases, myoglobin, and catalase showed reversible FeIII/FeII voltammetry on pyrolytic graphite electrodes and catalytic current for hydrogen peroxide and oxygen. Amperometric responses for these films to H2O2 at 0 V are likely to contain significant contributions from catalytic reduction ...

a hydroponics study was carried out in order to understand the behavior of antioxidant enzymes, soluble protein content and its effect on cell death in different old leaves of durum wheat. two month after planting, the different old leaves were sampled and the activity of super oxide dismutase (sod), catalase, ascorbat peroxidase and guaiacol peroxidase were measured. furthermore, the rate of c...

The catalase reaction of catalase-peroxidases involves catalase-specific features built into a peroxidase core. An arginine, 20 A from the active-site heme, acts as a molecular switch moving between two conformations, one that activates heme oxidation and one that activates oxoferryl heme reduction by H(2)O(2), facilitating the catalatic pathway in a peroxidase. The influence of the arginine is...

In order to survey effect of salinity and cadmium on catalase and peroxidase enzymes activity , proline accumulation, Malondialdehyde and protein content variation in  Brassica oleracea an experiment was conducted in green house condition factorial design based on completely random block in 2014 year. The first factor was cadmium at four levels (0, 50, 100 and 200 ppm) and second factor was sal...