Hypoxic ischemic (HI) injury in neonates damages brain tissues. We examined the mechanism of hyperbaric oxygen preconditioning (HBO-PC) in neonatal HI rat model. Seven-day-old rat pups were subjected to left common carotid artery ligation and hypoxia (8% oxygen at 37 degrees C) for 90 min. HBO (100% O(2), 2.5 atmospheres absolute for 2.5 h) were administered by placing pups in a chamber 24 h be...

cladribine acts as an antineoplastic, immunosuppressive and anti-inflammatory agent. the aim of this study was to examine the possible impact of this drug on the epidermis of wistar rats. the control group received standard feed and water. the experimental group i was treated with cladribine in a schema corresponding to the treatment of multiple sclerosis in humans. the material for microscopic...

econazole nitrate (en), a synthetic compound, is now in use as a routine antifungal drug. en was shown to have antitumor effect, the tumor cell killing mechanisms, however, remain unclear. in this research, the apoptosis-inducing effect of en on mcf-7 cells was investigated. the results showed that en inhibited the proliferation of mcf-7 cells in a time- and dose-dependent manner by mtt method ...

Proteolytic processing is required for the activation of most caspases. However, recent reports have suggested that the activation of the mammalian initiator caspase caspase-9 occurs during dimerization rather than after processing. Previously, we reported that, in normal living Drosophila S2 cells, the initiator caspase Dronc is continuously processed to a 40 kDa form we called Pr1 and that, d...

Murine erythroleukemia cells were subjected to physicochemical stresses such as high pressure (60--110 MPa), heating (42--45 degrees C), and ultraviolet (UV) irradiation (5--25 kJ/cm(2)). After exposure to these stresses, the cells were cultured at 37 degrees C and atmospheric pressure. The number of the cells treated at 100 MPa, 45 degrees C, or 20 kJ/cm(2) remained constant or decreased at an...

Pore-forming toxins (PFT) form the largest family of secreted toxins from pathogenic bacteria. Staphylococcus aureus produces different hemolysins, and α-hemolysin is one of the well-studied PFTs for which the X-ray structure of the pore is available. These toxins form heptameric pores of 1–2 nm in size in cell membranes, leading to various outcomes, including apoptotic cell death, in the host ...

406 Emerging Infectious Diseases • Vol. 9, No. 3, March 2003 Correction, Vol. 8, No. 12 In the article "Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway" by Joo-Sung Yang, et al., errors occurred in the Figure 5 caption on page 1384: delta symbols inadvertantly dropped or were replaced by the letter "d." The corrected caption appears below and online h...

Background: Using natural compounds with low toxicity on normal cells and high efficacy on malignant cells is highly appreciated for treatment of colorectal cancer (CRC). In the present study, the effect of fish-oil derived eicosapentaenoic acid (EPA) on the cell number, cell proliferation rate and caspase-3 enzyme activity in LS174T human colorectal cancer cell line was investigated. Methods:...

A series of novel β-carboline 1,3,4-oxadiazole derivatives were designed and synthesized, and the in vitro cytotoxic activity against Sf9 cells and growth inhibitory activity against Spodoptera litura were evaluated. Bioassay results showed that most of these compounds exhibited excellent in vitro cytotoxic activity. Especially, compound 37 displayed the best efficacy in vitro (IC50 = 3.93 μM),...