Papain was used to prepare a casein hydrolysate with a degree of hydrolysis of 9.4 %. The hydrolysate had the in vitro antioxidant activity with a DPPH radical scavenging activity of 38.7 % and an EC50 of 1.63 mg/mL. Extrinsic phenylalanine or tyrosine was added to the hydrolysate for a papain-catalyzed plastein reaction. The temperature, substrate mass per volume fraction, and the levels of en...

The putative effects of a tryptic bovine alphaS1-casein hydrolysate on stress-induced sleep disorders were investigated and their possible link with typical blood stress parameters such as plasma corticosterone concentrations and glycaemia was assessed. Rats were subjected to chronic stress in the form of environmental disturbances, while receiving an oral administration of the alphaS1-casein h...

Casein and soy protein were digested by papain to three degrees of hydrolysis (DH) 7.3%-13.3%, to obtain respective six casein and soy protein hydrolysates, aiming to clarify their in vitro proliferation and anti-apoptosis towards a human osteoblastic cell line (hFOB1.19 cells). Six casein and soy protein hydrolysates at five levels (0.01-0.2 mg/mL) mostly showed proliferation as positive 17β-e...

Background Breastfeeding is the gold standard of infant feeding, however not all infants with cow’s milk protein allergy (CMPA) tolerate human milk. In these cases, it seems recommended the use of extensively hydrolyzed formula (eHF). Preliminary analyses such as antigenicity and protein quality evaluation are needed to ensure that new formula will be nutritionally suitable, tolerated and safe ...

BACKGROUND Multiple food intolerance in infants, including intolerance to extensively hydrolysed proteins (HP), is often difficult to treat. However, few data have been reported on clinical outcome and dietary treatment of these patients. AIMS To evaluate the clinical characteristics of patients with HP-intolerance and the long-term outcome of treatment with ass' milk. PATIENTS AND METHODS ...

Rumen and caecal contents, obtained from slaughterhouse cattle and rumen contents obtained from a fistulated wether were incubated in vitro with ground hay in the presence and absence of, respectively, casein hydrolysate and mucin. Differences in stoichiometry of rumen and caecal fermentations, indicative of reductive acetogenesis in the caecum, were confirmed, except for incubations with free ...

The amino acids in an acid hydrolysate of casein were catabolized more extensively by Megasphaera elsdenii than those in an enzymic hydrolysate. Threonine and serine were most actively degraded, but no resultant increase in growth yield occurred. Branched-chain volatile fatty acid production, which increased as the dilution rate of a glucose-limited chemostat decreased, seemed to be associated ...

The acquisition and assimilation strategies followed by members of the infant gut microbiota to retrieve nitrogen from the gut lumen are still largely unknown. In particular, no information on these metabolic processes is available regarding bifidobacteria, which are among the first microbial colonizers of the human intestine. Here, evaluation of amino acid auxotrophy and prototrophy of Bifidob...

A recent study by Lacroix et al (1) compared the postprandial utilization of nitrogen from 3 different proteins, namely micellar casein (“slow protein”), milk soluble protein isolate (“fast protein”), and total milk protein (another “slow protein”). Their data suggest that slow protein induces better postprandial nitrogen utilization than does fast protein. According to Lacroix et al, “This res...

We compare the effects of a moderate hypocaloric, high-protein diet and resistance training, using two different protein supplements, versus hypocaloric diet alone on body compositional changes in overweight police officers. A randomized, prospective 12-week study was performed comparing the changes in body composition produced by three different treatment modalities in three study groups. One ...