Light is important for the production of metabolites by plant cell culture. It is therefore necessary to evaluate the light conditions with respect to exposure time. Both enhancement and inhibition have been observed depending on exposure time and species. Callus culture of D. nervosa was established and effect of light and passage on cardiac glycoside production was studied. In vitro cultures ...

This study investigated the effects of cardiac glycosides on single-channel activity of the cardiac sarcoplasmic reticulum (SR) Ca2+ release channels or ryanodine receptor (RyR2) channels and how this action might contribute to their inotropic and/or toxic actions. Heavy SR vesicles isolated from canine left ventricle were fused with artificial planar lipid bilayers to measure single RyR2 chann...

In the present study, an attempt was made to investigate the anti-bacterial activity of Nyctanthes arbortristis, Nerium oleander and Catharathus roseus. The crude drug powder extracts of the leaves of the above plants were taken for the study. The antibacterial activity was performed by using both gram positive and gram gative organism viz., B.subtilis and E.coli respectively. The Phytochemical...

Cardiac glycosides such as digoxin are Na+/K+-ATPase inhibitors that are widely used for the treatment of chronic heart failure and cardiac arrhythmias; however, recent epidemiological studies have suggested a relationship between digoxin treatment and increased mortality. We previously showed that nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) ...

Cardiac glycosides as inhibitors of the sodium/potassium adenosine triphosphatase (sodium pump) have been reported to block cancer growth by inducing G2/M phase arrest in many cancer cells. However, no detailed studies have been performed to distinguish between these two phases of cardiac glycoside-arrested cells. Furthermore, the underlying mechanisms involved in this cell cycle arrest process...

Several therapeutic compounds have been identified that prolong the QT interval on the electrocardiogram and cause torsade de pointes arrhythmias not by direct block of the cardiac potassium channel human ether-à-go-go-related gene (hERG) but via disruption of hERG trafficking to the cell surface membrane. One example of a clinically important compound class that potently inhibits hERG traffick...

BACKGROUND Cardiac glycosides are Na(+)/K(+)-pump inhibitors widely used to treat heart failure. They are also highly cytotoxic, and studies have suggested specific anti-tumor activity leading to current clinical trials in cancer patients. However, a definitive demonstration of this putative anti-cancer activity and the underlying molecular mechanism has remained elusive. METHODOLOGY/PRINCIPA...

Light is important for the production of metabolites by plant cell culture. It is therefore necessary to evaluate the light conditions with respect to exposure time. Both enhancement and inhibition have been observed depending on exposure time and species. Callus culture of D. nervosa was established and effect of light and passage on cardiac glycoside production was studied. In vitro cultures ...

The bushhopper Phymateus leprosus (Fabricius) in the field shows a special appetite for the milkweed Asclepias fruticosa. Asclepiadaceae, like Apocynaceae and Scrophulariaceae, contain cardiac glycosides. Raw and purified extracts of these plants phagostimulate larval and adult P. leprosus. We also screened natural and half-synthetic compounds found in those plant extracts. While saponins and s...