The purpose of this study was to examine the subcellular distribution and isoenzyme characteristics of carbonic anhydrase from the gills and respiratory air bladder of bowfin Amia calva, a primitive air-breathing fish. Separation of subcellular fractions by differential centrifugation revealed that the vast majority of carbonic anhydrase from the gills of bowfin originated from the cytoplasmic ...

Turtles possess a significant postcapillary CO2 partial pressure (PCO2) disequilibrium between arterial blood and alveolar gas. There are several possible explanations for this blood disequilibrium including a slow rate of erythrocyte physiological anion shift (Cl-/HCO3- exchange) or inaccessibility of plasma HCO3- to red blood cell or pulmonary carbonic anhydrase. The present study characteriz...

Binary Logistic Regression (BLR) has been developed as non-linear models to establish quantitative structure- activity relationships (QSAR) between structural descriptors and biochemical activity of carbonic anhydrase inhibitors. Using a training set consisted of 21 compounds with known ki values, the model was trained and tested to solve two-class problems as active or inactive on the basi...

PURPOSE Carbonic anhydrases play a central buffering role in current models of fluid transport in corneal endothelium, but in humans, clinical use of carbonic anhydrase inhibitors (CAIs) for the management of glaucoma does not cause corneal swelling. This study compares species differences in response to CAIs in human versus bovine corneal endothelial transport. METHODS Short-circuit current ...

Reaction of aromatic/heterocyclic sulfonamides containing a free amino group with triflic anhydride afforded compounds possessing trifluoromethanesulfonamido moieties in their molecule. The Zn(II) and Cu(II) complexes of these new sulfonamides were prepared and characterized by standard procedures (elemental analysis, spectroscopic, magnetic, thermogravimetric and conductimetric measurements). ...

Immobilization of Carbonic Anhydrase for Biomimetic CO2 Capture Maria Elena Russo, Stefania Scialla, Viviana De Luca, Clemente Capasso, Giuseppe Olivieri, Antonio Marzocchella a Istituto di Ricerche sulla Combustione– Consiglio Nazionale delle Ricerche, P.le V. Tecchio 80, 80125 Napoli, Italy b Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli St...

Acute hypoxic pulmonary vasoconstriction can be inhibited by high doses of the carbonic anhydrase inhibitor acetazolamide. This study aimed to determine whether acetazolamide is effective at dosing relevant to human use at high altitude and to investigate whether its efficacy against hypoxic pulmonary vasoconstriction is dependent on carbonic anhydrase inhibition by testing other potent heteroc...

The enzyme carbonic anhydrase appears to be a central molecular component in the suite of physiological and biochemical adaptations to low salinity found in euryhaline crustaceans. It is present in high activities in the organs responsible for osmotic and ionic regulation, the gills, and more specifically, the individual gills that are specialized for active ion uptake from dilute sea water. Wi...

1. Erythrocyte carbonic anhydrase I was measured by immunoelectrophoresis in blood samples taken at intervals during the menstrual cycle of 26 normal women. Plasma progesterone concentrations were also measured in 18 of these subjects. In every case, the erythrocyte carbonic anhydrase I concentration reached a maximum at that point in the cycle when there was maximum secretion of progesterone. ...