Many insect and fungal pathogens posing agronomically important threats specifically target the roots in strawberry. The use of a root-specific promoter to confer expressionof resistancegenes in a targetedmanner has the potential appreciably to benefit the genetic improvement of commercial strawberry varieties. A novel gene, FaRB7, was isolated from strawberry (Fragaria3 ananassa Duch.) and fou...

We describe a system for gene expression in plants based on the regulation mechanism of the yeast metallothionein (MT) gene. The system consists of two elements: (i) the yeast ace1 (activating copper-MT expression) gene encoding a transcription factor under control of the cauliflower mosaic virus (CaMV) 35S RNA promoter, and (ii) a gene of interest under control of a chimeric promoter consistin...

Cotton leaf curl Burewala virus (CLCuBuV), belonging to the genus Begomovirus, possesses single-stranded monopartite DNA genome. The bidirectional promoters representing Rep and coat protein (CP) genes of CLCuBuV were characterized and their efficacy was assayed. Rep and CP promoters of CLCuBuV and 35S promoter of Cauliflower mosaic virus (CaMV) were fused with β-glucuronidase (GUS) and green f...

Many insect and fungal pathogens posing agronomically important threats specifically target the roots in strawberry. The use of a root-specific promoter to confer expression of resistance genes in a targeted manner has the potential appreciably to benefit the genetic improvement of commercial strawberry varieties. A novel gene, FaRB7, was isolated from strawberry (Fragariaxananassa Duch.) and f...

An improved version of the previously obtained cloning vector pCass was constructed by partially duplicating the 35S promoter used to drive the transient transcription of cloned viral cDNAs. Full-length cDNAs of the three genomic RNAs of tomato aspermy cucumovirus (TAV) cloned in this improved pCass (designated pCass2) gave a 3-fold higher infectivity in two plant species tested than the same c...

Lines. All Arabidopsis thaliana lines were in the Columbia background. The pin1 mutant allele used was pin1-7. DR5::GFP refers to the DR5rev::GFP line (1), in which the DR5 promoter consists of nine repeats of the DR5 element (2), fused in inverse orientation to the minimal CaMV 35S promoter and the coding sequence of an endoplasmic-reticulumtargeted eGFP protein. This construct was kindly prov...

In order to evaluate the functions of putative transcriptional regulatory regions of cotton genes, the upstream regulatory sequences (promoters) of two cotton genes, Gh-sp (seed protein) and Gh-rbcS (ribulose-1,5-bisphosphate carboxylase small subunit), were fused with a bb-glucuronidase (GUS) reporter gene and the cauliflower mosaic virus (CaMV) 35S terminator. These two gene constructs were t...

Potato ( Solanum tuberosum L.) cultivar IPB CP1 is suitable as a raw material for the potato chip industry. plants are sensitive to various abiotic stresses such drought, aluminium and salinity, which induce reactive oxygen species (ROS). ROS very toxic plant cells. Superoxide dismutase (SOD) one of enzymes that catalyse H2O2 O2. This study aimed establish transgenic cv. containing MmCuZn‐SOD g...