C-CAM (Cell-CAM 105) is a cell surface glycoprotein that is involved in cell-cell adhesion of rat hepatocytes in vitro. To elucidate the adhesion mechanism the binding properties of purified C-CAM were investigated. Using proteins immobilized on nitrocellulose it was found that radiolabeled C-CAM bound to C-CAM but not to a variety of other proteins. Partitioning in Triton X-114 showed that C-C...

Background and Aim: Chairside computer-aided design/computer-aided manufacturing (CAD/CAM) systems have become considerably more accurate, reliable, efficient, fast, and prevalent since 1985 when CEREC was introduced. The inceptive restorative material option for chairside CAD/CAM restorations was limited to ceramic blocks. Today, restorative material options have been multiplied and include me...

In the past 10 years evidence - based medicine (EBM) has developed well in all aspects of allopathic medicine giving a new dimension to patient care including cost reduction and attention to medical ethics. With the public and medical community return to complementary and alternative medicine (CAM) in the last two decades there has been a call for evidence - based CAM … in the same way that all...

In the past 10 years evidence - based medicine (EBM) has developed well in all aspects of allopathic medicine giving a new dimension to patient care including cost reduction and attention to medical ethics. With the public and medical community return to complementary and alternative medicine (CAM) in the last two decades there has been a call for evidence - based CAM … in the same way that all...

A number of point mutations in the intracellular Ca2+-sensing protein calmodulin (CaM) are arrhythmogenic, yet their underlying mechanisms are not clear. These mutations generally decrease Ca2+ binding to CaM and impair inhibition of CaM-regulated Ca2+ channels like the cardiac Ca2+ release channel (ryanodine receptor, RyR2), and it appears that attenuated CaM Ca2+ binding correlates with impai...

background and aims. cad/cam systems facilitate the use of zirconia substructure materials for all-ceramic copings. this in vitro study investigated the marginal and internal fit of zirconia copings made with cad/cam system and slip-casting method. materials and methods. sixteen cad/cam made zirconia copings and 16 slip-cast made zirconia copings were fabricated and cemented with glass-ionomer ...

BACKGROUND Calmodulin (CaM) mutations have been identified recently in subjects with congenital long QT syndrome (LQTS) or catecholaminergic polymorphic ventricular tachycardia (CPVT), but the mechanisms responsible for these divergent arrhythmia-susceptibility syndromes in this context are unknown. We tested the hypothesis that LQTS-associated CaM mutants disrupt Ca2+ homeostasis in developing...

Autophosphorylation of alpha-Ca(2+)/calmodulin-dependent protein kinase II (CaM kinase II) at Thr(286) results in calmodulin (CaM) trapping, a >10,000-fold decrease in the dissociation rate of CaM from the enzyme. Here we present the first site-directed mutagenesis study on the dissociation of the high affinity complex between CaM and full-length CaM kinase II. We measured dissociation kinetics...