PIWOWARCZYK BARBARA, PINDEL ANNA, MUSZYŃSKA EWA. 2016. Callus Induction and Rhizogenesis in Lathyrus sativus L. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(1): 123–128. Grass pea (Lathyrus sativus L.) is a leguminous plant distinguished by great resistance to abiotic and biotic stresses that can become a valuable source of protein feed in the nearest future. Howev...

This study aimed to clarify the relationship between the mechanical environment at the fracture site and endogenous fibroblast growth factor-2 (FGF-2). We compared two types of fracture healing with different callus formations and cellular events using MouseFix(TM) plate fixation systems for murine fracture models. Left femoral fractures were induced in 72 ten-week-old mice and then fixed with ...

Interest in the medicinal properties of secondary metabolites of Boesenbergia rotunda (fingerroot ginger) has led to investigations into tissue culture of this plant. In this study, we profiled its primary and secondary metabolites, as well as hormones of embryogenic and non-embryogenic (dry and watery) callus and shoot base, Ultra Performance Liquid Chromatography-Mass Spectrometry together wi...

 Stevia rebaudiana Bert (Asteraceae) is herbaceous plant with sweet leaves, which are the source of sweetener products. The present study focuses on effects of different combination of plant growth regulators on callus induction, callus cell growth and regeneration and volatile constituents from callus of stevia in tissue culture condition. The results of present study showed callus formation p...

Genotypic variation in cytokinin oxidase has been detected in enzyme preparations from Phaseolus vulgaris L. cv Great Northern and Phaseolus lunatus L. cv Kingston callus cultures. Although cytokinin oxidase preparations from Great Northern and Kingston callus tissues appear to have very similar substrate specificities, the cytokinin oxidase activities from the two callus tissues were found to ...

Follow-up radiographs are important to assess proper alignment and adequate healing of a fracture. The formation of a callus is one characteristic used to follow these features, but the timing and size of its appearance is variable. Therefore, when to expect to see a callus on radiographs requires a general understanding of the fracture healing process and the many variables that can affect cal...

Objective: Our objective is to observe and detect the facture healing, the change of Bone Mineral Density (BMD), and the expressions of Alkaline Phosphatase (ALP), Osteocalcin (OC), Bone Morphogenetic Protein (BMP)-2, Insulin Growth Factor (IGF)-1 and collagen type 1 in local fracture site and serum, and explore the factors that affect the fracture healing in diabetic rats. Methods: 60 male rat...

Callus formation is a critical step for successful fracture healing. Little is known about the molecular composition and mineral structure of the newly formed tissue in the callus. The aim was to evaluate the feasibility of small angle x-ray scattering (SAXS) to assess mineral structure of callus and cortical bone and if it could provide complementary information with the compositional analyses...

We analyzed tissue and cells from a stationary and a rapidly growing hyperplastic callus from a patient with osteogenesis imperfecta (OI) type IV and compared the results with those of compact bone and skin fibroblasts of an age-matched control. Collagen and protein contents per cell were low in the callus tissues and collagen I and III were overmodified as evidenced by an elevated level of hyd...

The habituation of soybean (Glycine max) callus can be induced rapidly, by exposing the tissue to small amounts (10(-9)molar) of compounds including 2.4-dinitrophenol and phenoxyisobutyric acid for brief periods of time. Such compounds reportedly exhibit antiauxin activity. Various soybean callus phenotypes have been isolated which continue to exhibit hormone habituation 14 months following the...