Agrobacterium-mediated transformation of indica rice has been established in only a limited number of cultivars because the regeneration of plants from transformed embryogenic calli is highly cultivar-specific. Establishment of a highly efficient plant regeneration system from shoot apex explants applicable to many cultivars of indica rice will accelerate the application of transformation techn...

Rye is second only to wheat among grains most widely used in the making of bread and is also a very important gene resource for breeding and improvement of wheat and other cereal crops owing to tolerance to abiotic stress factors such as low temperatures, drought and poor soil conditions. However, application of biotechnologies has been limited in rye breeding since it is one of the most recalc...

The human herpes simplex virus thymidine kinase type 1 gene (HSVtk) acts as a conditional lethal marker in mammalian cells. The HSVtk-encoded enzyme is able to phosphorylate certain nucleoside analogs (e.g. ganciclovir, an antiherpetic drug), thus converting them to toxic DNA replication inhibitors. The utility of HSVtk as a conditional negative-selection marker was explored in Arabidopsis thal...

l h e human herpes simplex virus thymidine kinase type 1 gene (HSVtk) acts as a conditional lethal marker in mammalian cells. The HSVtk-encoded enzyme is able to phosphorylate certain nucleoside analogs (e.g. ganciclovir, an antiherpetic drug), thus converting them to toxic DNA replication inhibitors. l h e utility of HSVtk as a conditional negative-selection marker was explored in Arabidopsis ...

Efficient procedures are outlined for plant regeneration through direct shoot bud formation and indirect organogenesis through callus formation in Ornithogalum virens Lindl using bulb scale as explant. Murashige and Skoog’s (MS) medium containing 1 mg/L (5.4mM) NAA and 2 mg/L (4.4mM) BA was most effective in direct induction of shoot buds from explant. Callus cultures were raised from the bulb ...

Tissue culture is an attractive alternative for plant propagation. Micropropagation is a technique to ensure a constant and uniform source of ornamental plants. Matthiola incana is an important ornamental species mainly cultivate by seed. Matthiola incana seeds were germinated on solid MS medium without plant growth regulators. Shoot proliferation and root formation are possible using kinetin (...

Differentiation and morphogenetic potential of Allium commutatum Guss. callus tissue was investigated in five subcultures. Callus tissue was derived from root tips of in vitro grown seedlings and maintained on MS medium supplemented with 3% sucrose, 0.8% agar, 4.5 pM 2,4-D and 4.6 pM kinetin. Pieces of calli were subcultured on media with different concentrations and combinations of 2,4-D (1.0,...

Background Agrobacterium mediated gene transfer technology offers the potential to introduce novel, high-value traits into selected, elite tree genotypes. Significant progress has been made in the regeneration and genetic transformation of Eucalyptus trees, but transformation efficiency has generally been low for eucalypts [1], especially for elite clonal genotypes. As a preliminary step toward...

Interest in the medicinal properties of secondary metabolites of Boesenbergia rotunda (fingerroot ginger) has led to investigations into tissue culture of this plant. In this study, we profiled its primary and secondary metabolites, as well as hormones of embryogenic and non-embryogenic (dry and watery) callus and shoot base, Ultra Performance Liquid Chromatography-Mass Spectrometry together wi...

explants of leaves, petals, anthers, styles and ovaries of c. speciosus, c. cancellatus, c. gilanicus and c. almehensis were cultured on ls media with three different combinations of naa and bap in the dark. callus and neoformant organs (leaf and corm-like structure and ordinary and contractileroots) were fomd on some of these explants. corm explants from these species were also cultured on ms ...