نتایج جستجو برای: caco 2 cell line

تعداد نتایج: 4062388  

Journal: :Journal of virology 2000
A Esclatine M Lemullois A L Servin A M Quero M Geniteau-Legendre

Human cytomegalovirus (CMV) causes severe disease in immunosuppressed patients and notably infects the gastrointestinal tract. To understand the interaction of CMV with intestinal epithelial cells, which are highly susceptible to CMV infection in vivo, we used the intestinal epithelial cell line Caco-2 and demonstrated that CMV enters predominantly through the basolateral surface of polarized C...

2014
Rukhsanda Aziz M T Rafiq Jie Yang Di Liu Lingli Lu Zhenli He M K Daud Tingqiang Li Xiaoe Yang

Cadmium (Cd) is a widespread environmental toxic contaminant, which causes serious health-related problems. In this study, human intestinal cell line (Caco-2 cells) and normal human liver cell line (HL-7702 cells) were used to investigate the toxicity and bioavailability of Cd to both cell lines and to validate these cell lines as in vitro models for studying Cd accumulation and toxicity in hum...

Journal: :Oncology reports 2006
Yoshio Araki Hiroyuki Sugihara Takanori Hattori

Pathogenic mechanisms responsible for inflammatory bowel disease (IBD) are poorly understood. In an IBD animal model, the oral administration of polysaccharides such as dextran sulfate sodium (DSS) induces colitis, which exhibit several clinical and histological features for IBD. However, pathogenic factors in the development of colitis remain unclear. Therefore, we investigated possible mechan...

Journal: :Alternatives to laboratory animals : ATLA 2005
Flavia Zucco Anne-Françoise Batto Giovanna Bises Jean Chambaz Arianna Chiusolo Rosa Consalvo Heide Cross Gianni Dal Negro Isabella de Angelis Gérard Fabre François Guillou Sebastian Hoffman Loïc Laplanche Etienne Morel Martine Pinçon-Raymond Pilar Prieto Laura Turco Giulia Ranaldi Monique Rousset Yula Sambuy Maria Laura Scarino François Torreilles Annalaura Stammati

Differentiated human intestinal Caco-2 cells are frequently used in toxicology and pharmacology as in vitro models for studies on intestinal barrier functions. Since several discrepancies exist among the different lines and clones of Caco-2 cells, comparison of the results obtained and optimisation of models for use for regulatory purposes are particularly difficult, especially with respect to ...

Journal: :فصلنامه علمی پژوهشی گیاهان دارویی 0
f hadjiakhoondi department of pharmacognosy and medicinal plants research center, faculty of pharmacy, tehran university of medical sciences sn ostad department of toxicology and pharmacology, faculty of pharmacy, tehran university of medical sciences m khanavi department of pharmacognosy and medicinal plants research center, faculty of pharmacy, tehran university of medical sciences a hadjiakhoondi department of pharmacognosy and medicinal plants research center, faculty of pharmacy, tehran university of medical sciences, tehran, iran tel & fax: +98 - 21 - 66483626 b farahanikia department of pharmacognosy and medicinal plants research center, faculty of pharmacy, tehran university of medical sciences a salarytabar department of pharmacognosy and medicinal plants research center, faculty of pharmacy, tehran university of medical sciences

background: numerous molecules in papaveraceae family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a few cases. objective: numerous molecules in this family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a few...

1999
CHAOBIN HU

Hu, Chaobin, and David H. Perlmutter. Regulation of a1-antitrypsin gene expression in human intestinal epithelial cell line Caco-2 by HNF-1a and HNF-4. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G1181–G1194, 1999.— There is still relatively limited information about mechanisms of gene expression in enterocytes and mechanisms by which gene expression is regulated during enterocyte di...

Background and Objectives: In the original in vitro method for evaluation of iron bioavailability using caco-2 cell model, ferritin formation by cells is considered as a functional indicator of both absorption and utilization of iron. However, as iron is measured in the tested material and then ferritin concentration is measured in caco-2 cell lysate, it is almost impossible to have a concrete ...

Journal: :American journal of physiology. Cell physiology 2004
John McLaughlin Philip J Padfield Julian P H Burt Catherine A O'Neill

On interaction with the intestine, the mycotoxin ochratoxin A is know to cause rapid inflammation, diarrhea, and increased bacterial translocation. All these effects are consistent with a decrease in epithelial barrier function. However, this has not been shown directly. We determined that ochratoxin A is able to reduce the barrier properties of the model intestinal cell line Caco-2. Over 24 h,...

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