We tested the hypothesis that, due to greater hepatic free fatty acid (FFA) load, portal delivery of FFAs, as in visceral obesity, induces hyperinsulinemia and increases endogenous glucose production to a greater extent than peripheral FFA delivery. For 5 h, 10 microeq.kg(-1).min(-1) portal oleate (n = 6), equidose peripheral oleate (n = 5), or saline (n = 6) were given intravenously to conscio...

Investigation of phosphofructokinase in normal and regenerating livers led to the discovery of an inactivating factor in the extracts of these livers. The inactivating factor was found to be a mixture of free fatty acids. The fatty acid compositions of the normal and regenerating livers are the same, but the concentrations of most of the fatty acids are at least 3 to 4 times higher in the latte...

Previous work has shown that gluconeogenesis from a variety of substrates is stimulated by oleate. This paper reports data on the effect of oleate on tissue levels of coenzyme A derivatives, ketone bodies, citric acid cycle intermediates, and adenine nucleotides in rat livers perfused with alanine, lactate, or pyruvate. The effect of oleate on the activity of the citric acid cycle with lactate ...

OBJECTIVE High circulating free fatty acids, commonly associated with obesity and insulin resistance, impair structure and function of the microvasculature. However, the mechanisms by which fatty acids cause microvascular remodeling are unclear. Using the mesangial cell model of microvascular pericytes, we demonstrate that the monounsaturated free fatty acid oleate induces a myofibroblast pheno...

PURPOSE To understand various soft contact lens materials' ability to adsorb common tear lipids. METHODS Ten unworn polymers of nine types were individually soaked in 1.0 mL of 1.75 μg/mL cholesterol oleate or 1.0 mL of 0.5 μg/mL phosphatidylcholine solutions for 1 or 14 days. The adsorbed lipids were extracted with chloroform-methanol, which underwent assay quantification for cholesterol ole...

Previous work has shown that gluconeogenesis from a variety of substrates is stimulated by oleate. This paper reports data on the effect of oleate on tissue levels of coenzyme A derivatives, ketone bodies, citric acid cycle intermediates, and adenine nucleotides in rat livers perfused with alanine, lactate, or pyruvate. The effect of oleate on the activity of the citric acid cycle with lactate ...

The size distribution of new vesicles formed after addition of oleate in different forms to preformed egg yolk phosphatidylcholine (EggPC) vesicles was studied by gel exclusion chromatography. The addition of oleate to preformed vesicles resulted in the formation of new small vesicles. Fission of preformed vesicles incorporated by oleate and partial solubilization of the vesicles by addition of...

Free fatty acids have been reported to induce cell death (lipotoxicity), but the effects depend on the carbon chain length and number of double bonds. Medium-chain saturated fatty acids (MC-SFAs), such as laurate, have less lipotoxicity than long-chain saturated fatty acids (LC-SFAs), such as palmitate. Monounsaturated fatty acids, such as oleate, have also been reported not only to exert cytot...

Oleate-dependent phospholipase D (PLD; EC 3.1.4.4) has been reported in animal systems, but its molecular nature is unkown. Multiple PLDs have been characterized in plants, but none of the previously cloned PLDs exhibits the oleate-activated activity. Here, we describe the biochemical and molecular identification and characterization of an oleate-activated PLD in Arabidopsis. This PLD, designat...

Bitumen emulsions (BE) are widely used in road construction, for building and structure waterproofing. Storage stability is one of the main BE requirements. They should only break on contact with surface. The sodium oleate effect as a surfactant anionic bitumen waterproofing purposes has been studied. increased storage have developed. optimal content oleate, providing high complex emulsion resu...