A reliable solid-phase extraction – high-performance liquid chromatography (SPE-HPLC) method for the determination of flavonoids in European Bupleurum species B. baldense Turra and B. affine Sadler was developed. Extraction of aerial parts by ultrasound with 80% aqueous methanol and fractionation of flavonoids by means of SPE allowed a good recovery of analytes between 99 and 104%. The subseque...

An antiulcer polysaccharide fraction (BR-2) from Bupleurum falcatum L. was examined for its effect on the healing of chronic ulcers induced by acetic acid in rats. When BR-2 was administered orally to the rats, it was shown to be effective in the healing of acetic acid-induced chronic ulcer. This result suggests that the use of herbal prescriptions containing B. falcatum L. may prove useful for...

Some interesting Bupleurum (Apiaceae) specimens with very branched stems, (1–)2-rayed inflorescences and ivory-white bracteoles were collected from Tepebaşı District (Eskişehir Province). The specimes, at first glance, resembled the pendikum (sect. Aristata) species, which is endemic in Türkiye, as a habit. However, result of detailed examination, it was determined that there some differences. ...

BACKGROUND Bupleurum polysaccharides (BPs), isolated from Bupleurum smithii var. parvifolium, possesses immunomodulatory activity, particularly on inflammation. Bacterial endotoxin lipopolysaccharide (LPS) triggers innate immune responses through Toll-like receptor 4 (TLR4) on host cell membrane. The present study was performed to evaluate whether the therapeutic efficacy of BPs on suppression ...

Diabetes mellitus is related to low-grade chronic inflammation and oxidative stress. Bupleurum Polysaccharides (BPs), isolated from Bupleurum smithii var. parvifolium has anti-inflammatory and anti-oxidative properties. However, little is known about its therapeutic effects on diabetes. In this experiment, the effects of BPs on alleviation of diabetes and the underlying mechanisms were investig...

Two new lignans (R,Z)-3-(3,4-dimethoxybenzyl)-2-(3, 4-dimethoxybenzylidene)-gamma-butyrolactone (1) and (2R, 3S)-2-acetoxy-2,3-bis(3,4-dimethoxybenzyl)-gamma-butyrolactone (2), and four new polyacetylenes, (2Z,8E,10E)-heptadeca-2,8,10-triene-4, 6-diyn-1-ol (3), (2E,9Z)-octadeca-2,9-diene-4,6-diyne-1,18-diol (4), (2E,9Z)-18-hydroxy-octadeca-2,9-diene-4,6-diyn-1-yl acetate (5), and (2E,9Z)-1-hydr...

In this study, caffeic acid (CA) and its three derivatives including 3-caffeoylquinic acid (3-CQA, neochlorogenic acid), 4-caffeoylquinic acid (4-CQA, cryptochlorogenic acid), and 5-caffeoylquinic acid (5-CQA, chlorogenic acid) were identified in Bupleurum chinense aerial parts using reverse-phase high-performance liquid chromatography (RP-HPLC) with photodiode array (PDA) detector, reference c...