Background: This study examined the effect of including one of two growth factors (100 ng/ml IGF-1 or 20 ng/ ml EGF) in combination with one of two antioxidants (50 μM cysteamine or 50 μM beta-mercaptoethanol) in invitro maturation of buffalo oocytes, followed by fertilization and subsequent development of embryos. Materials and Methods: The oocytes were recovered by in vivo ovum pick-up techni...

The knowledge of oocyte activation and somatic cell nuclear transfer in the swamp buffalo (Buballus bubalis) is extremely rare. The objectives of this study were the following: (1) to investigate the various activation treatments on the parthenogenetic development of buffalo oocytes, (2) to examine the events of nuclear remodeling and in the in vitro development of cloned buffalo embryos recons...

Follicular fluid (FF) accumulates in the antrum of the ovarian follicle and provides the microenvironment for oocyte development. FF plays an important role in follicle growth and oocyte maturation. The FF provides a unique window to investigate the processes occurring during buffalo follicular development. The observed low quality of buffalo oocytes may arise from the poor follicular microenvi...

the aim of this study was to determine the quality of cumulus-oocyte-complexes (cocs) and the effects of bovine serum albumin (bsa) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. cocs were collected from slaughterhouse buffalo ovaries by aspiration method. only normal grades cocs were matured for 48 hours in tcm-199 media. two groups were created: one for the ...

Assisted reproductive technologies such as artificial insemination (AI) and multiple ovulation and embryo transfer (MOET) have been introduced to overcome reproductive inefficiencies and accelerate genetic gain. However, MOET in water buffalo is compromised by the poor ovulatory response of this animal species to hormonal treatment (Karaivanov 1986; Karaivanov et al. 1990; Misra et al. 1990; Cr...

The objective of this study was to investigate the effect photoperiod and other environmental factors on population Water buffalo oocytes in Veracruz, Mexico. Oocytes (n=123) were obtained by follicular aspiration graded according morphological characteristics. Data regarding regional (temperature, humidity, daylight) collected from a historic database metrological station. Statistical analysis...

The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...

AIM The present study was carried out to evaluate the effect of supplementation of β-mercaptoethanol (β-ME) on in vitro maturation rate and glutathione (GSH) level of buffalo oocytes. MATERIALS AND METHODS Oocytes were recovered from buffalo's ovaries collected from government approved slaughter house (near Kamela darwaza, Surat) of Surat Municipal Corporation. The obtained oocytes were in vi...