The principal objective of this study was to derive an improved procedure for cryopreservation of swamp buffalo (Bubalus bubalis) spermatozoa. Experiments were conducted to determine effects of cooling rate, intermediate plunge temperature and warming rate on motility and acrosome integrity of spermatozoa. Spermatozoa were obtained from three bulls (three ejaculates/bull) and were subjected to ...

AIM To test the hypothesis that season affects the semen quality of swamp buffalo (Bubalus bubalis) bulls used for artificial insemination (AI) under tropical conditions in Thailand, as it does in Bos taurus and Bos indicus. METHODS Clinical and andrological examinations, and monitoring of semen production and quality were carried out on five mature, healthy swamp buffalo AI bulls in Thailand...

The functional integrity of spermatozoa in various domestic animals like horse, cattle and swine is evaluated by hypo osmotic swelling test. The hypo osmotic swelling test has not been tested in the fresh semen of Nili-Ravi buffalo bull. The aim of the present study was evaluation of membrane integrity by establishing the best hypo osmotic swelling solution. Ten solutions with different osmolar...

The artifi cial insemination (AI) technique plays an important role in improvement of conception rate, prevention of sexually transmitted diseases and transmission of genetic material to the next generation. The breeding soundness evaluation (BSE) plays an important role in selection of male buffalo for the said purposes. This evaluation is an effective, inexpensive and easy method for selectio...

This study was conducted to assess the post thaw quality of Kundhi buffalo semen diluted in tris based diluents. After collection the ejaculates were pooled and checked for color, volume, pH, mass activity, progressive linear motility% (PLM), and intactness of cell membrane. The semen qualifying these tests was divided into four aliquots A, B, C and D, diluted with tris based diluents; the last...