In chemistry, biology, medical sciences and pharmaceutical industries, many reactions have to be checked by transporting and mixing expensive liquids. For such purposes, microfluidics systems consisting of closed channels with external pumps have been useful. However, the usage has been limited because of high fabrication cost and need for a fixed setup. Here, we show that open-capillary channe...

Four simple and sensitive visible spectrophotometric methods have been developed for the assay of dutasteride. Method I describes the interaction of dutasteride, as an n-electron donor, with chloranil, as a pi-acceptor, in acetonitrile to give reddish orange colored chloranil radical anion with absorption maxima at 525 nm. Method II involves the oxidative coupling reaction of dutasteride with 3...

The design, fabrication and testing of a photolithographically fabricated, glass-based multireflection absorbance cell for microfluidic devices, in particular microchip-based capillary electrophoresis (CE) systems is described. A multireflection cell was fabricated lithographically using a three-mask process to pattern aluminum mirrors above and below a flow channel in a chip, with 30 microm di...

1. The renal excretion of phenol red and other phenolsulphophthalein dyes (bromophenol blue and bromothymol blue) was studied in clearance experiments on anaesthetized rabbits. 2. Net tubular excretion of phenol red reached a maximal value of 8 mumole/min at a plasma concentration of ultrafiltrable dye of about 0.1 mM and was decreased at higher plasma concentrations. Decreases in net tubular e...

Degeneration of cultivated strains of Flammulina velutipes is a serious problem. We developed a simple colorimetric method to detect degenerate strains by using a liquid medium supplemented with bromothymol blue and lactose. The ability of a strain to develop normal mushrooms could be determined by the color of the medium.

Antisera prepared against the eight recognized serotypes of Ureaplasma urealyticum were tested against the homologous and heterologous antigens by a modified metabolic inhibition test that used bromothymol blue broth in microtiter plates. The method gives clear endpoint determinations which are usually maintained on continued incubation. Antisera against serotype 8 showed cross-reactions with t...

Five laboratory-adapted strains of Ureaplasma urealyticum showed comparable colony counts when incubated in eight gaseous environments including air, in different concentrations of carbon dioxide, and under anaerobiosis. For primary isolation on genital mycoplasma agar 95% N2-5% CO2 gave 100% correlation with growth in bromothymol blue broth, whereas certain strains failed to grow on agar incub...

Chromosphere such as triaryl methane family of dyes used in various industries can affect the body living organism system upon interaction with most biochemical molecule is DNA. In this research, binding mode chromophore that Bromothymol blue, Bromophenol Bromocresol green and Malachite double strand DNA phosphate buffer at pH 6.9 was investigated through UV/VIS spectroscopy. The study shows bl...