Dicentric chromosomes undergo a breakage-fusion-bridge cycle as a consequence of having two centromeres on the same chromatid attach to opposite spindle poles in mitosis. Suppression of dicentric chromosome breakage reflects loss of kinetochore function at the kinetochore-microtubule or the kinetochore-DNA interface. Using a conditionally functional dicentric chromosome in vivo, we demonstrate ...

LEE, SUNG WON. A Probability Model for Random Fiber Breakage. (Under the direction of JOHN WILLIAM BISHlR). Two fundamental questions in connection with the random breakage of fibers in a breakage system are set forth: Given a fiber group of any particular length, (1) What is the probability that a fiber of the length groups breaks at all? (2) What is the probability that a fiber breaks into s ...

In process engineering, the breakage behavior of particles is needed for the modeling and optimization of comminution processes. A popular tool to describe (dynamic) processes is population balance modeling (PBM), which captures the statistical distribution of particle properties and their evolution over time. It has been suggested previously to split up the description of breakage into a machi...

Detection of tool breakage is of vital importance in automated manufacturing. Various methods have been attempted, and it is considered that the use of discrete wavelet transform (DWT), which is much more efficient and just as accurate wavelet analysis, may provide a realistic solution to the detection of tool breakage in operation. The DWT uses an analyzing wavelet function which is localized ...

Lactococcus lactis 61-14 isolated from river water produced a bacteriocin active against a wide range of Gram-positive bacteria. N-terminal amino acid sequencing, mass spectral analysis of the purified bacteriocin, and genetic analysis using nisin-specific primers showed that the bacteriocin was a new natural nisin variant, termed nisin Q. Nisin Q and nisin A differ in four amino acids in the m...

Programmed chromosome breakage occurs at 50-200 specific sites in the genome of Tetrahymena thermo-phila during somatic nuclear (macronuclear) differentiation. Previous studies have identified a 15 bp sequence, the Cbs (for chromosome breakage sequence), that is necessary and sufficient to specify these sites. In this study we determined the effects of mutations in the Cbs on its ability to spe...

Male genitalia are among the fastest evolving morphological characters, and at a general level sexual selection seems to be involved. But experimental determination of the functions of many remarkable genitalic elaborations is very rare. Here we present the first study to address experimentally the adaptive function of a male genital structure that is not involved in sperm transfer. Females of ...

Tetrahymena telomeres are protected by a protein complex composed of Pot1, Tpt1, Pat1, and Pat2. Pot1 binds the 3' overhang and serves multiple roles in telomere maintenance. Here we describe Pot2, a paralog of Pot1 which has evolved a novel function during Tetrahymena sexual reproduction. Pot2 is unnecessary for telomere maintenance during vegetative growth, as the telomere structure is unaffe...

the log damage and value loss were examined by felling and bucking function, species, and damage type, in kheyrud forest in namkhaneh district in the hyrcanian forest of iran. in order to calculate the wood value following tree felling and bucking, 250 trees and 167 logs consisting of beech and hornbeam species were measured. there were five types of damage recorded following the tree felling o...