Porcine intestinal spirochaetes are fastidious anaerobic organisms and, as a consequence, it has been necessary to develop various protocols to enhance their isolation from or detection in faeces. Immunomagnetic separation (IMS) is a method developed recently to improve separation of target cells from mixed cell suspensions. The purpose of the present study was to compare the relative sensitivi...

The spirochetes Brachyspira hyodysenteriae and B. pilosicoli are pig intestinal pathogens that are the causative agents of swine dysentery (SD) and porcine intestinal spirochaetosis (PIS), respectively. Although some inactivated bacterin and recombinant vaccines have been explored as prophylactic treatments against these species, no effective vaccine is yet available. Immunoproteomics approache...

A novel TaqMan 5-plex real-time PCR was developed for the identification of Brachyspira species in swine. B. hyodysenteriae, pilosicoli, and suanatina are identified using three hydrolysis probes targeting cpn60, while hampsonii is recognized by another nox specific probe. This highly sensitive assay easy to implement routine veterinary diagnostic laboratories enables rapid differentiation betw...

avian intestinal spirochaetosis (ais) is a condition arising from colonization of the caeca and colon of birds with anaerobic spirochaetes of the genus brchyspira. the purpose of the present study was to evaluate pcr amplification from formalin-fixed, paraffin-embedded tissue method for detecting infected laying hens with brachyspira pilosicoli  with the same primers that have been used for det...

Human intestinal spirochetosis (HIS) is associated with overgrowth of the large intestine by spirochetes of the genus Brachyspira. The microbiological diagnosis of HIS is hampered by the fastidious nature and slow growth of Brachyspira spp. In clinical practice, HIS is diagnosed histopathologically, and a significant portion of cases may be missed. Fluorescence in situ hybridization (FISH) is a...

BACKGROUND Brachyspira associated diarrhea is a re-emerging concern for Canadian swine producers. To identify critical control points for reducing the impact of Brachyspira on production, improved diagnostic tools and a better understanding of the on-farm epidemiology of these pathogens are required. A cross-sectional study was conducted for the detection of Brachyspira on a commercial, two-sit...

Feral pigs are recognized as being a potential reservoir of pathogenic microorganisms that can infect domestic pigs and other species. The aim of this study was to investigate whether feral pigs in Western Australia were colonized by the pathogenic enteric bacteria Lawsonia intracellularis, Brachyspira hyodysenteriae and/or Brachyspira pilosicoli. A total of 222 feral pigs from three study-popu...