This study was designed to investigate the effects of applying 1 mT static magnetic field (SMF) during the vitrification process, on the viability of ovarian follicles after vitrification-warming and autotransplantation. The study was conducted in two phases. In the first phase, ovaries of female NMRI mice (6 to 8 weeks old) were randomly divided into three groups: 1- Freshly isolated ovaries f...

Franz Leydig first described the testicular cells in 1850 that now bear his name. For the next 50 yr after their discovery, Leydig cells were the subject of further studies by light microscopy, and diverse speculations were offered about their possible function. In 1903, Pol Bouin and Paul Ancel provided the first substantial evidence that Leydig cells constituted an endocrine gland controlling...

Antigens, preparation of lymph nodes and culture media, stimulation in vitro, implantation in clotted plasma, incubation, and estimation of antibody t.iters on fluid withdrawn at 3 day intervals, were similar to those in the accompanying paper (1). In most of these experiments, the fragments were exposed to antigen by incubation before implantation into plasma. Histological Methods for Fluoresc...

Diabetic cardiomyopathy contributes to the high incidence of mortality in both types of diabetes. We aimed to investigate the histochemical aspects of collagen and reticular fibers in the cardiac muscle and evaluate the influence of physical exercise on these aspects. Wistar rats were divided in 4 groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic...

The questions considered in this paper are, " Does the mammalian ~esticle, in addition to the formation of sperm, furnish an internal secretion essential to the normal development of the organism?" and, " I f so, which cells of the testicle have this function ?" The testicle is well suited for such a study, both from its anatomic relations and from its histologic structure. It is easily accessi...

Especially for neuroscience and the development of new biomarkers, a direct correlation between in vivo imaging and histology is essential. However, this comparison is hampered by deformation and shrinkage of tissue samples caused by fixation, dehydration and paraffin embedding. We used magnetic resonance (MR) imaging and computed tomography (CT) imaging to analyze the degree of shrinkage on mu...

With a view to checking the presence of melatonin in the pineal gland of the cow, in the present work we used six adult animals, ranging in age from one to six years, which were sacrificed at dawn. Sections of 6 micro m thickness of Bouin-fixed and paraffin-embedded pineal glands were incubated in an anti-melatonin serum, which was provided by the Institute for Molecular and Cellular Recognitio...

Infertile men exhibit an aberrant protamine-1 (Prm1) to protamine-2 (Prm2) ratio at both the mRNA and protein level. We therefore investigated whether male infertility could be related to the amount of Prm1 and Prm2 mRNA by applying real time quantitative PCR following RNA extraction from routinely Bouin-fixed and paraffin-embedded testicular biopsies. Samples (n = 51) were normalized to the sa...

The structural changes in the uterine mucosa associated with the phenomena of estrus, menstruation, implantation of the embryo, pregnancy, and the puerperium have, for a long time, been of marked interest to the histologist. The staining reactions of the endometrium with Nile blue, Sudan III, Scharlach R, and osmic acid have indicated the possibility of a connection between the lipid content an...

Scientific assessment of harmful interactions of chemicals over the entire reproductive cycle are divided into three segments based on the period: from premating and mating to implantation (I), from implantation to major organogenesis (II), and late pregnancy and postnatal development (III). We combined the segments I and II to assess Plathymenia reticulata aqueous extract safety. In order to i...