Bone defects resulting from trauma, surgery, congenital malformations, and other factors are among the most common health problems nowadays. Although current strategies such as autografts allografts recognized successful treatments for stimulating bone regeneration, limitations graft source complications still exist. SmartBone® is a xeno-hybrid (made bovine matrix, poly(L-lactic-co-?-caprolacto...

Gelatin is a natural amphiphilic biopolymer that widely used in food products, pharmaceuticals, and cosmetics. We studied the effect of spray freeze drying on solubility amphiphilicity gelatin samples.
 The control sample was commercially produced edible gelatin. experimental samples were spray- freeze-dried gelatins obtained by enzymatic-acid hydrolysis cattle bone. Amino acid sequences d...

Introduction . Articular cartilage has a limited intrinsic capacity for repair, therefore, methods to repair or reconstruct osteochondral defects have been sought by many labs (). The search for the ideal cellular matrix has resulted in the development and testing of both artificial and natural scaffold materials(). Current cellular scaffolds, however, are limited by the presence of inflammator...

Background: Repair of bone defects is challenging for reconstructive and orthopedic surgeons. In this study, we aimedto histomorphometrically assess new bone formation in tibial bone defects filled with octacalcium phosphate (OCP),bone matrix gelatin (BMG), and a combination of both.Methods: A total of 96 male Sprague Dawley rats aged 6-8 weeks weighing 120-150 g were randomly allocatedinto thr...

Tissue engineering is a multidisciplinary field that combines cells, biomaterial scaffolds and environmental factors to achieve functional tissue repair. This thesis focuses on the use of macroporous gelatin microcarriers as scaffolds in tissue engineering applications, with a special focus on cartilage and bone formation by human adult cells in vitro. In our first study, human articular chondr...

BACKGROUND Matrix metalloproteinases (MMPs) degrade the extracellular matrix (ECM) and regulate remodeling and regeneration of bone. Enamel matrix derivative (EMD) protein has been used clinically for periodontal regeneration, although its molecular mechanisms are not clear. We evaluated the role of matrix metalloproteinases (MMPs) in regulating EMD-dependent degradation of gelatin on oeoblast-...

Calcium phosphate- (CaP-) based composite scaffolds have been used extensively for the bone regeneration in bone tissue engineering. Previously, we developed a biomimetic composite nanofibrous membrane of gelatin/β-tricalcium phosphate (TCP) and confirmed their biological activity in vitro and bone regeneration in vivo. However, how these composite nanofibers promote the osteogenic differentiat...

Cell-based tissue engineering using scaffolds provides a promising option for the repair of bone tissue damage caused by trauma or aging-related degeneration such as osteoporosis. In this study, a porous 3D scaffold was used to support the differentiation process of rat adipose -derived stem cells (ADSCs) into osteoblasts in vitro. The scaffold was made with chitosan, gelatin and chondroitin. I...