conclusions our findings showed that the treatment of flaps with bm-msc and aam transplantations significantly promoted flap survival compared to a control group. the viability of the flap was improved by combining bm-mscs with aam matrix scaffolds. materials and methods bm-mscs and aams were transplanted into subcutaneous tissue in the flap area. on the 7th postoperative day, the surviving fla...

Human mesenchymal stem cells (hMSCs), which also called mesenchymal stromal cells, are multipotent stem cell. Human MSCs typically are positive for the surface markers CD44, CD73, CD90, CD105, CD106, and also negative for hematopoietic markers CD34 and CD45.These cells can be isolated from postnatal bone marrow, adipose tissue, placenta, and scalp tissue, as well as from various fetal tissues. ...

bone marrow mesenchymal stem cells can differentiate into neurons and astrocytes after transplantation in the spinal cord injury of rats. although bone marrow mesenchymal stem cells are known to protect against spinal cord injury through anti-apoptotic effects, but generation of neural stem cells with large number and safety protocols unclear. in the present study, described the differentiation...

the aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. blastocyst stage embryos from pregnant nmri mice were obtained and cultured for 1-2 days in dmem medium. the inner cell masses formed...

introdution: stroke is one of the most common diseases caused by occlusion or rupture of blood vessels in brain. it brings heavily loads for families and societies. although some new strategies including treatment of tissue plasminogen activator have been applied in the clinic, these methods do not have perfect effect. accordingly, more effective therapeutic strategies need to be developed. thi...

introduction: transplantation of bone marrow stromal cells (bmscs) or schwann cells (scs) can increase axonal regeneration in peripheral nerve injuries. based on our previous investigations, the goal of the present work was to examine the individual and synergistic effects of the two different cell types in sciatic nerve injury . we pursued to evaluate the effects of bmscs and scs co-transplant...

We previously demonstrated that murine bone marrow stromal cells express high levels of cytochrome P4501B1 (CYP1B1) that metabolizes 7,12-dimethylbenza[a]anthracene (DMBA), and that DMBA activates the Ah receptor (AhR) in these cells in vitro. More recently, we reported that CYP1B1 is required for DMBA-induced lymphoblastoma formation in vivo. In this study, we addressed the hypothesis that bon...

The chemokine stromal cell-derived factor-1 (SDF-1) is constitutively expressed by bone marrow stromal cells and plays key roles in hematopoiesis. Fibroblast growth factor 2 (FGF2), a member of the FGF family that plays important roles in developmental morphogenic processes, is abnormally elevated in the bone marrow from patients with clonal myeloid disorders and other disorders where normal he...

The chemokine stromal cell–derived factor-1 (SDF-1) is constitutively expressed by bone marrow stromal cells and plays key roles in hematopoiesis. Fibroblast growth factor 2 (FGF2), a member of the FGF family that plays important roles in developmental morphogenic processes, is abnormally elevated in the bone marrow from patients with clonal myeloid disorders and other disorders where normal he...

Bone marrow stromal cells appear to be key regulatory elements in hematopoiesis and lymphopoiesis. These stromal cells respond to cytokine exposure and alter their pattern of hematopoietic growth factor production, suggesting a degree of functional plasticity. We examined the effect of two cytokines, interleukin-1 (IL-1) and IL-4, on stromal cell regulation of pre-B cell generation using the bo...