Interactions of the axial ligand with its blue copper center are known to be important in tuning spectroscopic and redox properties of cupredoxins. While conversion of the blue copper center with a weak axial ligand to a green copper center containing a medium strength axial ligand has been demonstrated in cupredoxins, converting the blue copper center to a red copper center with a strong axial...

Most of the present-day down-conversion white light-emitting devices (WLEDs) utilize rare-earth elements, which are expensive and facing the problem of shortage in supply. WLEDs based on the combination of orange and blue emitting copper nanoclusters are introduced, which are easy to produce and low in cost. Orange emitting Cu nanoclusters (NCs) are synthesized using glutathione as both the red...

Ibuprofen a non-steroidal anti-inflammatory drug (NSAID) is among the most prescribed drug on the world. Copper(II) complexes with NSAIDs are more active than their parent drugs and exhibit a lower toxicity than inorganic forms of copper [1] . Further, many of these complexes, including that of copper ibuprofenate complex, have a better anti-ulcer protective effect than the parent drugs. Binary...

In 1928 Krebs reported that the concentration of copper in the serum was significantly raised in the terminal stages of pregnancy. Since that time this observation has been confirmed by a number of workers (Gorter, Grendel, and Weyers, 1931; Locke, Main, and Rosbash, 1932; Sachs, Levine, and Fabian, 1935; Nielsen, 1944), who have shown that at full term the serum copper level is approximately t...

The plasmid-encoded pco copper resistance operon in Escherichia coli consists of seven genes that are expressed from two pco promoters in response to elevated copper; however, little is known about how they mediate resistance to excess environmental copper. Two of the genes encode the soluble periplasmic proteins PcoA and PcoC. We show here that inactivation of PcoC, and PcoA to a lesser extent...

We studied the coherent electron transport through metal–protein–metal junctions based on a blue copper azurin, in which ion was replaced by three different metal ions (Co, Ni and Zn). Our results show that neither protein structure nor transmission at Fermi level change significantly upon replacement. The discrepancy with previous experimental observations suggests mechanism taking place these...

Thin-layer spectroelectrochemical methods have been employed to measure the reduction potentials of the blue copper in Polyporus versicolor laccase (EC 1.10.3.2) between 7 degrees C and 41 degrees C (0.2 M sodium phosphate, pH 5.4). Thermodynamic parameters are: DeltaS degrees = -13.9 +/- 2 cal/mol-K; DeltaH degrees = -22.1 +/- 0.5 kcal/mol; E degrees (25 degrees C) = 780 +/- 3 mV vs. the norma...

Dissimilatory nitrite reductase was isolated from extracts of Alcaligenes xylosoxidans subsp. xylosoxidans (N.C.I.M.B. 11015), after activation of crude extracts by the addition of copper(II) sulphate. The enzyme was purified by a combination of (NH4)2SO4 fractionation and cationic-exchange chromatography to 93% homogeneity as judged by SDS/PAGE. SDS/PAGE and spray m.s. showed that the enzyme h...

Menkes disease is a fatal disease that can be induced by various mutations in the ATP7A gene, leading to unpaired uptake of dietary copper. The ATP7A gene encodes a copper(I)-translocating ATPase. Here the disease-causing A629P mutation, which occurs in the last of the six copper(I)-binding soluble domains of the ATPase (hereafter MNK6), was investigated. To understand why this apparently minor...

The electronic absorption, electronic circular dichroism and X-ray absorption spectroscopy of the blue copper protein plastocyanin is studied with density functional theory, time-dependent density functional theory and multireference configuration interaction in conjunction with classical molecular dynamics simulations. A strong correlation is observed between the excitation energy of the inten...