A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAM...

A novel method using ethanol was proposed for extracting lipids from wet microalga Picochlorum sp. at room temperature and pressure. In this study, Central Composite design (CCD) was applied to investigate the optimum conditions of lipid extraction. The results revealed that the solvent to biomass ratio had the largest effect on lipid extraction efficiency, followed by extraction time and tempe...

We propose a single-run liquid-chromatographic determination, with ultraviolet detection at 330 nm, for serum retinol and retinyl esters. The vitamin A derivatives are extracted according to the Bligh-Dyer procedure. With 200 microliter or serum, the lower detection limit is 50 microgram/liter for retinol and about 100 microgram/liter for retinyl esters. Within-run precision (CV) was 2.3% for r...

Palm oil mill effluent (POME) is a by-product of palm production. POME can be considered as harmful waste without any treatment. POME, however, has the potential to source low-cost carbon for lipid This study aims isolate oleaginous fungi from and evaluate ability production capacity purified isolates commercial strains (Aspergillus niger, Aspergillus oryzae Rhizopus spp.) POME. converted into ...

Accurate profiling of lipidomes relies upon the quantitative and unbiased recovery of lipid species from analyzed cells, fluids, or tissues and is usually achieved by two-phase extraction with chloroform. We demonstrated that methyl-tert-butyl ether (MTBE) extraction allows faster and cleaner lipid recovery and is well suited for automated shotgun profiling. Because of MTBE's low density, lipid...

Recently, a simple method for the extraction of lipids from wet biomass using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Here, the versatility of this method was evaluated for labyrinthula Aurantiochytrium limacinum (A. limacinum). The liquefied DME was passed through the extractor that filled by A. limacinum at different time intervals. The extract...

Objective: The objective of this study was to analyze fatty acids using Gas Chromatography-Mass Spectrometry (GC-MS) in combination with chemometric Principal Component Analysis (PCA) for the authentication Rattus norvegicus fat from other animal fats.
 Methods: Extraction raw meat norvegicus, beef, chicken, pork, and dogs Bligh Dyer method, then derivatized 0.2 N NaOCH3, precipitation sod...

Abstract This study aims to examine the effect of ultrasonic-assisted extraction method on yield oil and profile fatty acid from microalga yeast. The cultivated was identified genetically as Thraustochytrium multirudimentale MAST-1 yeast Rhodotorula mucilaginosa KAST-1. oils were extracted dried biomass by solvents using modified Bligh Dyer (BD) method. Before extraction, material underwent ult...

Indonesia is a country with majority Muslim population, and the halal food consumed should be assured. One type of that must considered from meat products. Due to price difference between non-halal meat, some unethical producers try adulterate beef rat meat. The research objective was employ FTIR spectroscopy in combination chemometrics for analysis adulteration meatballs. Lipid components meat...