Multidrug-resistant (MDR) Klebsiella pneumoniae is increasing worldwide with poorly characterised epidemiology in many parts of the world, particularly Africa. This study aimed to investigate molecular K. pneumoniae, identify diversity sequence types (ST), and detect carbapenem resistance genes major regional hospitals Khartoum, Sudan. isolates (n = 117) were cultured from four April 2015 Octob...

Due to annually recurring mass gatherings in Makkah, Saudi Arabia could be a hot spot for the collection of multidrug-resistant strains such as Pseudomonas aeruginosa (P. aeruginosa). The various genotypes of extended spectrum beta-lactamases (ESBLs) are SHV, TEM, CTX-M types VEB, PER, BEL-1, BES-1, ESBL enzymes in P. aeruginosa. The present study aimed to identify the prevalence these genes in...

Multidrug-resistant (MDR) Acinetobacter baumannii infections are of particular concern within medical treatment facilities, yet the gene assemblages that give rise to this phenotype remain poorly characterized. In this study, we tested 97 clinical A. baumannii isolates collected from military treatment facilities (MTFs) from 2003 to 2009 by using a molecular epidemiological approach that enable...

An investigation was carried out into the genetic mechanisms responsible for multidrug resistance in nine carbapenem-resistant Pseudomonas aeruginosa isolates from different hospitals in Recife, Brazil. Susceptibility to antimicrobial agents was determined by broth microdilution. Polymerase chain reaction (PCR) was employed to detect the presence of genes encoding β-lactamases, aminoglycoside-m...

Within the ESBL family the most common genes are temoneira (blaTEM) and sulfhydryl variable (blaSHV) with increasing reports of cefotaximase (blaCTX-M) [4]. The blaSHV and the blaTEM subgroups arise from mutational changes due to point mutations in the genes resulting in subtypes. Examples include blaSHV which differs from blaSHV-1 due to a single mutation of an amino acid from glycine to serin...

Escherichia coli clinical isolate BD07372 of sequence type ST131 recovered from a bed sore specimen exhibited high-level resistance to ceftazidime and cefotaxime but exhibited susceptibility to imipenem and meropenem. The isolate harbored two β-lactamase genes, the bla(CTX-M-15) gene carried by an ∼250-kbp plasmid carrying the FIA and FIC replicons and the bla(GES-5) gene carried by a class 1 i...