The impact of NaOH as a ballast water treatment (BWT) on microbial community diversity was assessed using the 16S rRNA gene based Ion Torrent sequencing with its new 400 base chemistry. Ballast water samples from a Great Lakes ship were collected from the intake and discharge of both control and NaOH (pH 12) treated tanks and were analyzed in duplicates. One set of duplicates was treated with t...

In this study, we validated the analytical performance of BRCA1/2 sequencing using Ion Torrent's new bench-top sequencer with amplicon panel with optimized bioinformatics pipelines. Using 43 samples that were previously validated by Illumina's MiSeq platform and/or by Sanger sequencing/multiplex ligation-dependent probe amplification, we amplified the target with the Oncomine™ BRCA Research Ass...

Detection of somatic mutations in non-small cell lung cancers (NSCLCs), especially adenocarcinomas, is important for directing patient care when targeted therapy is available. Here, we present our experience with genotyping NSCLC using the Ion Torrent Personal Genome Machine (PGM) and the AmpliSeq Cancer Hotspot Panel v2. We tested 453 NSCLC samples from 407 individual patients using the 50 gen...

Anti-P2P companies have begun to launch Internet attacks against BitTorrent swarms. We use passive and active Internet measurements to study how successful these attacks are at curtailing the distribution of targeted content. For our active measurements, we develop a crawler that contacts all the peers in any given torrent, determines whether leechers in the torrent are under attack, and identi...

Many alpine torrents convey regularly debris flows of high destructive hazard. After several granular events observed in Bruchji’s torrent near the Blatten village in the Swiss Alps, it was decided to manage the risk of similar debris flows by designing and constructing a control structure inside the torrent bed. Associated with a breach in the right bank side of the torrent, this control struc...

MOTIVATION The importance of fast and affordable DNA sequencing methods for current day life sciences, medicine and biotechnology is hard to overstate. A major player is Ion Torrent, a pyrosequencing-like technology which produces flowgrams--sequences of incorporation values--which are converted into nucleotide sequences by a base-calling algorithm. Because of its exploitation of ubiquitous sem...

A novel protocol for full-length Mycobacterium tuberculosis gene analysis of first- and second-line drug resistance was developed using the Ion Torrent Personal Genome Machine (PGM). Five genes-rpoB (rifampin), katG (isoniazid), pncA (pyrazinamide), gyrA (ofloxacin/fluoroquinolone), and rrs (aminoglycosides)-were amplified and sequenced, and results were compared to those obtained by genotypic ...

Multi-service residential access networks allow residential customers to choose amongst a variety of service offerings, over a range of Core Networks and subject to user requirements such as QoS, mobility, cost and availability. These issues place requirements on authentication for network access, with a need for mutual authentication of the residential gateway (RG) to the local access point (L...

Using C# as a parser, we process about 3.4 million pieces of data over 680 thousand torrents from thepiratebay.org, and create a graphical representation of the data by infographic. Info-graphic presents the information in an easily readable format, and also can be distributed across many webmediums. Based on the representation/analysis of the data, we are able to determine some interesting cha...