The rapidly growing field of biotechnology has created a critical need for simple, fast andhigh-throughput processes for the separation and purification of biomolecules from biologicalmatrices. In recent years, several bioseparation techniques have been proposed as advancedalternatives to the classical separation methods. These modern processes emphasize ultrahighselective and sensitive analysi...

During the past few years, nanoparticles have been used for various applications including, but not limited to, protein immobilization, bioseparation, environmental treatment, biomedical and bioengineering usage, and food analysis. Among all types of nanoparticles, superparamagnetic iron oxide nanoparticles, especially Fe3O4, have attracted a great deal of attention due to their unique magnetic...

Smart polymeric materials respond with a considerable change in their properties to small changes in their environment. Environmental stimuli include temperature, pH, chemicals, and light. "Smart" stimuli-sensitive materials can be either synthetic or natural. This review discusses the application of smart materials as tools to solve biological problems such as bioseparation, drug delivery, bio...

For the requirement of a high, fast and sufficient technology to suit the needs of 21st century biotechnology, the separation range of a microchip electrophoresis system was studied. Two DNA fragments on the human Y-chromosome, SY594 (82 bp) and 12f2 (88 bp), were successfully separated with a reproducibility of 1.9% and an accuracy of 2.8%. Then, a mixture of 10 DNA markers ranging from 61 bp ...

A new mode of transport is described that was capable of high-resolution separation of superparamagnetic materials from complex mixtures based on their size. Laminar flow and a rotating external magnetic field were applied to superparamagnetic beads assembled on a semiperiodic micromagnet array. Beads at the edge of the micromagnet array oscillated in-phase with the external magnetic field with...

A better understanding of protein behavior at solid-liquid interfaces is important to improve our knowledge of various natural and synthetic processes. A few examples where protein adsorption at a solid-liquid interface may play an important role are found in: blood coagulation, artificial kidney failure, biomedical microdevices, self-assembling microelectromechanical systems (MEMS), protein ba...

ger G and Albertsson PA (1983) AfRnity partitioning of phosphofructokinase from baker’s yeast using polymer-bound Cibacron blue F3GA. European Journal of Biochemistry 131: 589}594. KopperschlaK ger G and Birkenmeier G (1990) AfRnity partitioning and extraction of proteins. Bioseparation 1: 235}254. Tjerneld F, Johansson G and Joelsson M (1987) AfRnity liquid}liquid extraction of lactate dehydro...