The aim of this work was to discover the extent of interference by phosphoenolpyruvate (PEP) phosphatase in spectrophotometric assays of PEP carboxylase (EC 4.1.1.31) in crude extracts of plant organs. The presence of PEP phosphatase and lactate dehydrogenase (EC 1.1.1.27) in extracts leads to PEP-dependent NADH oxidation that is independent of PEP carboxylase activity, and hence to overestimat...

Gluten is a protein group found in wheat, barley, rye, and oats, known as cereals. When this vegetable introduced into the body, celiac disease can occurs. The use of bacterial fungal oligopeptidase to ensure cleavage gluten non-toxic fragments are considered promising alternative for disease. In study, Aspergillus niger Prolyl EndoPeptidase (AN-PEP) enzyme was cloned pET22b vector recombinantl...

Traditionally, impedance derived measures of cardiac autonomic balance (CAB) and regulation (CAR) are calculated using indices heart rate variability (HRV) that primarily reflect parasympathetic nervous system activity (e.g., high-frequency HRV | HF-HRV) pre-ejection period (PEP; a systolic time interval measure sympathetic activity). However, HF-HRV PEP considered chronotropic inotropic influe...

EPSP synthase is the target enzyme of glyphosate herbicides. Due to extensive use glyphosate, it very important obtain EPSPS genes with high resistance for development transgenic crops. GR79-EPSPS a class I certain isolated from glyphosate-contaminated soil. After more than 1000 generations, Y40I substitution was identified, and had nearly 1.8-fold decrease in Km [PEP] 1.7-fold increase Ki[glyp...

OBJECTIVE To assess and compare immediate effects of chest physiotherapy with positive expiratory pressure (PEP) versus oscillating PEP on transcutaneously measured blood-gas tensions in patients with cystic fibrosis. METHODS Fifteen patients (mean age 12.5 y, range 6.9-21.5 y) participated. The treatments were randomized and performed on 2 separate occasions, 8 weeks apart. Spirometry was co...

Silk fibroin (SF) peptide has been traditionally used as a treatment for flatulence, spasms, and phlegm. In this study, we examined whether SF peptide enhanced the antiinflammatory effect of PEP-1-FK506 binding protein (PEP-1-FK506BP) through comparing the anti-inflammatory activities of SF peptide and/or PEP-1-FK506BP. In the presence or absence of SF peptide, transduction levels of PEP-1-FK50...