The production, optimisation and partial characterisation of xylanases from newly isolated wild strains of Coprinellus disseminatus was performed in solid-state fermentation. Strains SH-1 and SH-2 showed high xylanase (727.78 and 227.99 IU/mL) with very low CMCase (0.925 and 0.660 IU/mL) and laccase (0.640 and 0.742 U/mL) activities at incubation time seven days, 37 degrees C and initial pH 6.4...

Lignocellulosic carbohydrates, i.e. cellulose and hemicellulose, have abundant potential as feedstock for production of biofuels and chemicals. However, these carbohydrates are generally infiltrated by lignin. Breakdown of the lignin barrier will alter lignocelluloses structures and make the carbohydrates accessible for more efficient bioconversion. White-rot fungi produce ligninolytic enzymes ...

An alkaliphilic Bacillus isolate (strain JAMB-750) was found to exoproduce a novel alkaline mannanase (AmA). AmA was purified to homogeneity (about 98% pure) as judged by SDS-polyacrylamide electrophoresis. The molecular mass was approximately 130 kDa. The hydrolysis patterns of various substrates indicated that the enzyme was an endo-type enzyme. The optimal temperature and pH for activity wer...

A thermoalkalophilic new species of Bacillus, similar to Bacillus arseniciselenatis DSM 15340, produced extracellular xylanase under solid state fermentation when wheat bran is used as carbon source. The extracellular xylanase was isolated by ammonium sulfate (80%) precipitation and purified using ion exchange chromatography. The molecular weight of xylanase was ~29.8 kDa. The optimum temperatu...

A xylan-degrading enzyme (endo ß-1,4 xylanase, EC 3.2.1.8) cleaves ß-1,4 glycosidic bond to produce xylose and is useful mainly in biobleaching paper pulp, pharmaceutical and food industries. The present investigation deals with the selection of derepressed mutant of Aspergillus niger GCBT-35 using antimetabolite 2-deoxy D-glucose (2DG) after N-methyl N-nitro N-nitroso guanidine (MNNG) treatmen...

Much progress has been made in the past several years concerning the biochemistry and molecular genetics of fungal wood decay. Aspects of this knowledge were obtained with the intent of using the fungi and their enzymes for selective and beneficial purposes; e.g., in biopulping and biobleaching of wood fiber, in bioremediation of pollutants, and in biomass conversions for fuel and chemical feed...

Beta-mannanase otherwise known as mannan endo-1,4-βmannosidase or 1,4-β-D-mannanase with an enzyme commission (EC) 3.2.1.78 breakdowns β-1,4-mannosidic bonds in the main chain of glucomannans, galactomannans and β-1,4-mannans [1]. It transforms the abundant mannan-rich heteromannan, glucomannan and galactomannan substrates into mannooligosaccharides [2] and a small amount of mannose, glucose an...

Microbial manganese peroxidases (MnPs) are ligninolytic enzymes primarily responsible for degrading lignin, but they also capable of dyes, organic pollutants, and emerging contaminants in wastewater. They have been isolated characterized from many white-rot fungi few bacteria. For microbial MnPs, the optimum pH range is between 3.5 9.0; temperature 25°C 70°C. Their molecular weights 25 kDa 68 k...