We evaluated long-term administration of estrogen after cardiac arrest and cardiopulmonary resuscitation (CA/CPR) on neurohistopathological and behavioral outcome. We also examined the effect of estrogen receptor (ER) stimulation using ER-alpha agonist propyl pyrazole triol (PPT) and ER-beta agonist diarylpropionitrile (DPN) on neuronal survival after CA/CPR to determine whether possible neurop...

Signals mediated by G-protein-linked receptors display agonist-induced attenuation and recovery involving both protein kinases and phosphatases. The role of protein kinases and phosphatases in agonist-induced attenuation and recovery of beta-adrenergic receptors was explored by two complementary approaches, antisense RNA suppression and co-immunoprecipitation of target elements. Protein phospha...

The orexin-1 receptor interacts with beta-arrestin-2 in an agonist-dependent manner. In HEK-293T cells, these two proteins became co-internalized into acidic endosomes. Truncations from the C-terminal tail did not prevent agonist-induced internalization of the orexin-1 receptor or alter the pathway of internalization, although such mutants failed to interact with beta-arrestin-2 in a sustained ...

The primary goal was to test the hypothesis that agonist-induced corticotropin-releasing factor type 1 (CRF(1)) receptor phosphorylation is required for beta-arrestins to translocate from cytosol to the cell membrane. We also sought to determine the relative importance to beta-arrestin recruitment of motifs in the CRF(1) receptor carboxyl terminus and third intracellular loop. beta-Arrestin-2 t...

OF THE MANY beta-receptor blocking agents developed over the past two decades only one, dl-propranolol, has become generally available to American physicians. However, as experience accumulates regarding the safety and efficacy of other beta-blockers, it is likely that at least some of these drugs (many of which are widely used in Europe) will gain approval for use in the United States. Thus an...

It is widely recognized that Th2 cytokines derived from T cells play a major role in the development of allergic lung inflammation that causes most asthma. Beta-agonists are important rescue and maintenance therapies for asthma, yet our understanding of beta-agonist effects on T cell biology is surprisingly poor. Recent studies using both cell culture and more integrative models are beginning t...

OF THE MANY beta-receptor blocking agents developed over the past two decades only one, dl-propranolol, has become generally available to American physicians. However, as experience accumulates regarding the safety and efficacy of other beta-blockers, it is likely that at least some of these drugs (many of which are widely used in Europe) will gain approval for use in the United States. Thus an...

Beta(2)-adrenergic receptors (beta(2)-AR) expressed on airway epithelial and smooth muscle cells regulate mucociliary clearance and relaxation and are the targets for beta-agonists in the treatment of obstructive lung disease. However, the clinical responses display extensive interindividual variability, which is not adequately explained by genetic variability in the 5'-flanking or coding regio...

OF THE MANY beta-receptor blocking agents developed over the past two decades only one, dl-propranolol, has become generally available to American physicians. However, as experience accumulates regarding the safety and efficacy of other beta-blockers, it is likely that at least some of these drugs (many of which are widely used in Europe) will gain approval for use in the United States. Thus an...