Adenine is widely used in clinical field, however, an excess of adenine is harmful. It is known that the feeding of an adenine-rich diet induces renal failure and decreases bone mineral density (BMD) and the serum testosterone level in male rats. However, there is little information about the influence of adenine on female animals. We compared the effects of adenine treatment between male and f...

APOBEC3G is a protein family that interferes with the replication of the HIV virus by altering the genomic sequence of the virus. To combat this, the virus evolves a countermeasure in the form of a protein called Viral Infectivity Factor (vif) binds to APOBEC to reduce its effectiveness. In vitro, dilutions from zero to 1mg of APOBEC3G are injected into HIV infected cells. The cells’ DNA sequen...

Guanylate cyclase is regulated by adenine nucleotides in membranes of intestinal mucosal cells. Basal guanylate cyclase was activated about twofold by adenine nucleotides. Activation was specific for adenine, as compared with the pyrimidine nucleotides UTP and CTP. In addition, enzyme activation was obtained in the presence of saturating concentrations of GTP, the substrate for guanylate cyclas...

Previous studies in our laboratory (1) have shown that intact ascites cells incorporate nicotinamide-14C into both nicotinamide adenine dinucleotide and nicotinic acid adenine dinucleotide. It was also shown that the specific activity of the nicotinamide adenine dinucleotide increased with increasing concentrations of nicotinamide-14C. High levels of nicotinic acid-r4C, on the other hand, did n...

The behaviour of adenine and 2’-deoxyadenosine-5’-monophosphate (dAMP) at positive surface potentials of a silver working electrode was investigated using surface-enhanced Raman scattering (SERS). The use of positive potentials in the presence of adenine or dAMP leads to a rapid accumulation of an intense spectrum. It is proposed that complexes of adenine (dAMP) with silver generate the observe...

An orphan G protein-coupled receptor from the rat has recently been demonstrated to act as a transmembrane receptor for the nucleobase adenine. The receptor is possibly involved in nociception. Here we report the cloning and functional expression of an additional G(i)-coupled receptor for adenine (Genbank accession code DQ386867). mRNA for this receptor was obtained from mouse brain and the mou...

Cobalt, nickel and copper complexes of adenine and guanine, as nucleic-acid constituents, were prepared. The adenine and guanine complexes are of tetrahedral and octahedral geometries, respectively. All are of high spin nature. The nickel complexes are of 2:1 metal:ligand ratio with Ni...Ni direct interaction in the guanine complex. The coordination bonds of adenine metal complexes are calculat...