Abstract Roasting is a crucial and essential step to produce quality coffee. However, it could lead the formation of toxic suspected carcinogenic or procancerogenic compounds, such as polycyclic aromatic hydrocarbons. In this work, simple easily automatable green procedure based on solid-phase microextraction coupled with gas chromatography for analysis acenaphthene, anthracene, benzo[ghi]peryl...

Sorption-desorption processes control soil-pollutant interactions. These processes determine the extent of pyrene transport in soils. Understanding sorption characteristics of pyrene is necessary in ascertaining its fate in soil. Laboratory batch experiments were conducted to study the sorptions-desorption of pyrene on eight soils from varying tropical agro-ecological zones (AEZs). The results ...

The object of this study was to investigate the formation of pyrene metabolites by the isopod Porcellio scaber as a possible tool in the environmental risk assessment of polycyclic aromatic hydrocarbon (PAH) exposure in terrestrial ecosystems. The formation of pyrene metabolites was studied after either pulse exposure to a single high dose, or prolonged exposure (14 d) to a lower dosage. Exposu...

The tumorigenic activities of benzo(e)pyrene, 9,10-dihydrobenzo(e)pyrene, 9,10-epoxy-9,10,11,12-tetrahydrobenzo(e)-pyrene, the diastereomeric bay-region 9,10-dihydroxy-11,12-epoxy-9,10,11,12-tetrahydrobenzo(e)pyrenes, and the K-region benzo(e)pyrene 4,5-oxide were assessed in newborn mice, Swiss-Webster mice received a total dose of 0.7 mumol of compound divided into three i.p. injections of 0....

The metabolism and covalent binding of the environmen tal carcinogen benzo(a)pyrene, a constituent of cigarette smoke, has been determined in isolated perfused lungs of shamand cigarette smoke-exposed and 3-methylcholanthrene-pretreated male Wistar rats. Rats were exposed to cigarette smoke for 1 hr daily for either 1 or 10 days. Benzo(a)pyrene (2 nmoles) was instilled intratracheally, and meta...

The metabolism and covalent binding of the environmen tal carcinogen benzo(a)pyrene, a constituent of cigarette smoke, has been determined in isolated perfused lungs of shamand cigarette smoke-exposed and 3-methylcholanthrene-pretreated male Wistar rats. Rats were exposed to cigarette smoke for 1 hr daily for either 1 or 10 days. Benzo(a)pyrene (2 nmoles) was instilled intratracheally, and meta...

Covalent binding of aromatic hydrocarbons to DNA is catalyzed by horseradish peroxidase with HzOz. Under optimal reaction conditions (30 PM hydrocarbon, 5 x lo-“ M HzOz, and 0.2 mg/ml of horseradish peroxidase at pH 7.0), binding was linear for 30 min. Binding of benzo[a]pyrene, 6-methylbenzo[a]pyrene, and 3-methylcholanthrene ranged from 40 to 100 pmol/mol of DNA. Fluorescence spectra of 6-met...

The metabolism of benzo(a)pyrene in cultured human colon has been investigated. Nontumorous colonie tissue was collected at the time of either surgery or "immediate autopsy" from patients with or without colonie cancer. After 24 hr in culture the expiants were exposed to [3H]benzo(a)pyrene for another 24 hr and the binding to cellular DMA and protein was measured. Two adducts, formed between be...

in this study, three stations of the north persian gulf were analyzed in order to determinethe effect of trophic levels on the concentration of harmful pahs in fish and sediment. in all cases,similar distributions were observed in which benzo(a)pyrene largely predominated andbenzo(b)fluoranthene and pyrene were the second major compounds in fish tissues. the predominantcomponents of pahs in sed...

The metabolism of 7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene results primarily in the ultimate carcinogenic metabolite of benzo[a]pyrene, 7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene and to a lesser extent 7,8-dihydroxy-9,10-dioxetane-7,8,9,10-tetrahydrobenzo[a]pyrene, from which chemiluminescence is observed. This specific microsomal chemiluminescence has been used to establish that t...