The metabolism of [Wlbenzo(a)pyrene with isolated hepatocytes from 3-methylcholanthrene-treated rats was examined with the aid of high pressure liquid chromatography. Covalent binding of [SHlbenzo(a)pyrene metabolites to the intracellular DNA was investigated. The effects of (Ynaphthoflavone, salicylamide, trichloropropene oxide, and diethylmaleate, individually or combined, on the metabolism a...

concentration levels of 16 polycyclic aromatic hydrocarbons (pahs) consisting of naphthalene, acenaphthylene, acenaphthene, fluorine, phenantherene, anthracene, fluor?anthene, pyrene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)p?yrene, dibenzo(a,h)ant?hracene, indeno(1,2,3-cd) pyrene and benzo(g,h,i)perylene were measured in water and sediment samples coll...

Uptake of benzo(a)pyrene by living cultured cells has been visualized in real time using digital fluorescence-imaging microscopy. Benzo(a)pyrene was noncovalently associated with lipoproteins, as a physiologic mode of presentation of the carcinogen to cells. When incubated with either human fibroblasts or murine P388D1 macrophages, benzo(a)pyrene uptake occurred in the absence of endocytosis, w...

The occurrence and levels of benzo[a]pyrene in various heat-treated foods from China were evaluated by high-performance liquid chromatography-fluorescence detection. In a total of 119 samples, 105 were found to contain benzo[a]pyrene at levels of 0.03 to 19.75 μg/kg. The benzo[a]pyrene contents in 12 animal source foods were higher than the Chinese maximum permissible level in food (5 μg/kg) an...

The metabolism and macromolecular binding of four metabolites of benzo(a)pyrene in hamster embryo fibroblasts has been studied. Two noncarcinogenic phenolic derivatives, 3-hydroxybenz(a)pyrene and 9-hydroxybenzo(a)pyrene, are rapidly metabolized, primarily to their respective glucuronic acid conjugates and other H2O-soluble conjugates (78.4 to 80.8% of total radioactivity). Water-soluble conjug...

background: residents of the northeastern region of iran experience high mortality rates from esophageal squamous cell carcinoma (escc). one etiologic factor might be exposure to high levels of polycyclic aromatic hydrocarbons, including benzo(|î±|)pyrene (bî±p). we examined whether food or water are the major sources of bî±p exposure in this population. materials and methods: we used a dietary...

during the last years, several exceedances of pm10 and benzo(a)pyrene limit values exceedances were recorded in taranto, a city in southern italy included in so-called areas at high risk of environmental crisis because of the presence of a heavy industrial district including the largest steel factory in europe. a study of these critical pollution events showed a close correlation with the wind ...

Crude oils and source rocks from the Northern and offshore depobelts of the Niger Delta basin, Nigeria have been characterized based on the distribution of pyrene and its derivatives by gas chromatography-mass spectrometry. Moreover, the crude oils have been characterized by the dominance of pyrene over fluoranthene while fluoranthene generally predominated over pyrene in the rock samples. Also...

Covalent binding of aromatic hydrocarbons to DNA is catalyzed by horseradish peroxidase with HzOz. Under optimal reaction conditions (30 PM hydrocarbon, 5 x lo-“ M HzOz, and 0.2 mg/ml of horseradish peroxidase at pH 7.0), binding was linear for 30 min. Binding of benzo[a]pyrene, 6-methylbenzo[a]pyrene, and 3-methylcholanthrene ranged from 40 to 100 pmol/mol of DNA. Fluorescence spectra of 6-met...

The evidence for biological involvement, the spectroscopic properties (especially EPR), and the reactions, of free radicals derived from benzo(a)pyrene and its methylated, hydroxylated, and fluorinated derivatives are reviewed.