This report describes novel baculovirus vectors designed to express mammalian beta1,4-galactosyltransferase and alpha2,6-sialyltransferase genes at early times after infection. Sf9 cells infected with these viral vectors, unlike cells infected with a wild-type baculovirus, produced a sialylated viral glycoprotein during the late phase of infection. Thus, the two mammalian glycosyltransferases e...

Removal of Ca2+ from the solution bathing neurons is known in many cases to alter the gating properties of voltage-dependent K+ channels and to induce a large, nonselective "leak" conductance. We used a heterologous expression system to test whether the leak conductance observed in neurons is mediated by voltage-dependent K+ channels in an altered, debased conformation. Voltage-dependent K+ cha...

M. (1992) Purification and characterization of human salivarygland prokallikrein from recombinant baculovirus-infected insect cells. Eur. J. Biochem. 206, 225–233. 6. Takada, Y., Skidgel, R. A., and Erdös, E. G. (1985) Purification of human urinary prokallikrein. Biochem. J. 232, 851–858. 7. Noda, Y., Takada, Y., and Erdös, E. G. (1985) Activation of human and rabbit prokallikrein by serine and...

Autographa californica nucleopolyhedrovirus (AcMNPV) does not replicate in Bombyx mori cells (Bm5, BmN). We have shown previously that when a short DNA sequence within AcMNPV ORF95, which encodes the viral helicase P143, is replaced with the colinear region of B. mori nucleopolyhedrovirus (BmNPV), AcMNPV gains the ability to replicate in Bm5 cells. To determine the mutational events in the p143...

Spodoptera litura dapat menyebabkan kehilangan hasil hingga 80% bahkan gagal panen. Nuclear Polyhedrosis Virus merupakan agensi hayati dari kelompok patogen serangga famili Baculoviridae yang banyak digunakan sebagai agens dan (SlNPV) salah satu pemanfaatan NPV efektif dalam mengendalikan litura. Kendala penggunaan SlNPV yaitu tidak tahan terhadap sinar matahari sehingga perlu bahan pelindung a...

47 samples from the Martignoni baculovirus collection were characterized by PCR amplification of the lef-8 gene. This led to the identification of sequences from viruses that either were not present in the database, or had been identified, but not further characterized. These included an NPV and a GV from Pseudoletia (Mythimna) unipuncta, and NPVs from Coloradia pandora, the oak and hemlock loo...

Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insec...

A template-dependent, in vitro rotavirus RNA replication system was established. The system initiated and synthesized full-length double-stranded RNAs on rotavirus positive-sense template RNAs. Native rotavirus mRNAs or in vitro transcripts, with bona fide 3' and 5' termini, derived from rotavirus cDNAs functioned as templates. Replicase activity was associated with a subviral particle containi...