The complete annotated genome sequence of Bacillus megaterium bacteriophage Slash is described here. Several key features related to morphogenesis, replication/recombination, DNA metabolism, and lysis are described. Slash also encodes a homolog of SleB, a germination-specific cell wall amidase.

INETY TWO local bacterial isolates, from the rhizosphere and ...... soil around the root system of bean (Vicia faba) grown in Kalubia Governorate in Egypt, were bio-prospected for polyhydroxyalkanoate (PHA) accumulation. Three isolates accumulated ≥ 20 % of PHAs, they were identified as Pseudomonas fluorescens S48, Bacillus megaterium 7A and B. megaterium UBF19. The tested isolates gave the max...

Photomicrographic evidence of reversion of Bacillus megaterium protoplasts to the bacillary form on soft agar plates hypertonic medium is demonstrated.

AIMS To determine the mechanism of wet heat killing of spores of Bacillus cereus and Bacillus megaterium. METHODS AND RESULTS Bacillus cereus and B. megaterium spores wet heat-killed 82-99% gave two bands on equilibrium density gradient centrifugation. The lighter band was absent from spores that were not heat-treated and increased in intensity upon increased heating times. These spores lacke...

Bacillus megaterium, an industrial strain, has been widely used in protein production and the vitamin C industry. Here we reported a finished, annotated, and compared 4.14-Mbp high-quality genome sequence of B. megaterium WSH-002, which is the companion strain for Ketogulonicigenium vulgare in the vitamin C industry and is stocked in our laboratory.

A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His6-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signal peptide, and recovered by one-step affinity chromatography.

Bacillus megaterium is a Gram-positive, spore-forming saprophytic inhabitant of diverse environments. It is a reservoir for industrial chemical production and is emerging as a model organism for studying sporulation and protein localization. Here, we introduce the complete genome of Page, a novel podophage infecting B. megaterium.

Previous work demonstrated that Bacillus megaterium QM B1551 spores that are null for the sleB and cwlJ genes, which encode cortex-lytic enzymes (CLEs), either of which is required for efficient cortex hydrolysis in Bacillus spores, could germinate efficiently when complemented with a plasmid-borne copy of ypeB plus the nonlytic portion of sleB encoding the N-terminal domain of SleB (sleB(N)). ...

A key enzyme for the biosynthesis and bioengineering of heparin, 3-O-sulfotransferase-1 (3-OST-1), was expressed and purified in Gram-positive Bacillus subtilis and Bacillus megaterium. Western blotting, protein sequence analysis, and enzyme activity measurement confirmed the expression. The enzymatic activity of 3-OST-1 expressed in Bacillus species were found to be similar to those found when...

Wachsman, J. T. (University of Illinois, Urbana) and R. Mangalo. Use of 8-azaguanine for the isolation of auxotrophic mutants of Bacillus megaterium. J. Bacteriol. 83:35-37. 1962.-8-Azaguanine is bactericidal for Bacillus megaterium strain KM, primarily under conditions where growth is possible. This analogue has been used to enrich for auxotrophic mutants in ultraviolet-irradiated populations....