Bacterial ribosomal protein S7 initiates the folding of the 3' major domain of 16S ribosomal RNA by binding to its lower half. The X-ray structure of protein S7 from thermophilic bacteria was recently solved and found to be a modular structure, consisting of an alpha-helical domain with a beta-ribbon extension. To gain further insights into its interaction with rRNA, we cloned the S7 gene from ...

The 'Number Needed to Treat' (NNT) is a useful measure for estimating the number of patients that would need to receive a therapeutic intervention to avoid one of the adverse events that the treatment is designed to prevent. We explored the possibility of an adaption of NNT to estimate the 'Number Needed to $ave' (NN$) as a new, conceptual systems metric to estimate potential cost-savings to th...

Experimental Details S3 General Considerations S3 Synthesis of 2 S3 Synthesis of 3 S4 Synthesis of 4 S4 Synthesis of 5 S5 Synthesis of 6 S6 Synthesis of 6-HD S6 Synthesis of 7 S6 Synthesis of 8 S7 Reaction of 8 with D2 S7 Reactivity with AB Analogs S7 Time Resolved AB Dehydrogenation Experiments S8 NH2BH2 Trapping Experiments S8 Eudiometric Measurement Details S8 Figure S1—Eudiometer Diagram S9...

In this study, we used site-directed mutagenesis to disrupt an interaction that had been detected between ribosomal proteins S7 and S11 in the crystal structure of the bacterial 30 S subunit. This interaction, which is located in the E site, connects the head of the 30 S subunit to the platform and is involved in the formation of the exit channel through which passes the 30 S-bound messenger RN...

We evaluated the effects of frequency of energy supplementation on growth and measurements of innate and humoral immune responses of preconditioning beef steers following vaccination. Angus steers ( = 24; 221 ± 6.3 kg; 177 ± 4 d of age) were weaned on d -7 and kept in a single drylot pen with free access to tall fescue hay and concentrate DMI at 0.5% of BW (50:50 mix of soyhulls and corn gluten...

Sequence determination of full-length cDNA clones of genomic segments 7-10 (S7-S10) of oat sterile dwarf fijivirus (OSDV) revealed that the 5' and 3' ends of the plus strands of these segments had the same conserved terminal sequences, 5' AACGAAAAA and UUUUUUUAGUC 3'. These sequences are similar, but not identical, to the conserved terminal nucleotide sequences of the genomic segments of rice b...

The proteins encoded by rice black streaked dwarf fijivirus (RBSDV) genomic segments 7-10 (S7-S10) were characterized. Open reading frames (ORFs) from these segments were expressed as fusion proteins in Escherichia coli. Antibodies raised against the expressed products were used as probes to determine whether the viral ORFs encode structural proteins. In Western blots, antibodies to the express...

Quantitative analysis by high-pressure liquid chromatography shows that all commercial samples of elemental sulfur besides S8 contain traces of S7 (up to 0.56%) and in some cases also S6, S9, and polymeric sulfur (S«,). Pure S8 can be obtained by recrystallization from CS2. Elemental sulfur minerals also quite often contain traces of S7 (up to 0.3%). Since neither irradiation by daylight at 20 ...