Live attenuated Salmonella are attractive vaccine candidates for mucosal application because they induce both mucosal immune responses and systematic immune responses. After breaking the epithelium barrier, Salmonella typhimurium is found within dendritic cells (DC) in the Peyer's patches. Although there are abundant data on the interaction of S. typhimurium with murine epithelial cells, macrop...

The facultative intracellular pathogen Salmonella enterica resides in a specific membrane-bound compartment termed the Salmonella-containing vacuole (SCV). Despite being segregated from access to metabolites in the host cell cytosol, Salmonella is able to efficiently proliferate within the SCV. We set out to unravel the nutritional supply of Salmonella in the SCV with focus on amino acids. We s...

Colanic acid (CA) is a common exopolysaccharide produced by many genera in the Enterobacteriaceae. It is critical for biofilm formation on HEp-2 cells and on chicken intestinal tissue by Salmonella. In this study, we generated different CA synthesis gene mutants and evaluated the immune responses induced by these mutants. One of these mutations, Δ(wza-wcaM)8, which deleted the whole operon for ...

Salmonella enterica is a versatile live carrier for the presentation of recombinant vaccine antigens. Fusion proteins of a type III secretion system effector and heterologous vaccine antigens can be translocated by live attenuated Salmonella strains and mediate protective immunity against infections. Here we investigated the use expression cassettes for translocated fusion protein consisting of...

Salmonella DNA adenine methylase (Dam) mutants that lack or overproduce Dam are highly attenuated for virulence in mice and confer protection against murine typhoid fever. To determine whether vaccines based on Dam are efficacious in poultry, a Salmonella Dam(-) vaccine was evaluated in the protection of chicken broilers against oral challenge with homologous and heterologous Salmonella serovar...

The antibody response of mice to a smooth strain of Salmonella typhimurium was shown previously to be extremely rapid and potent. As measured by the complement-mediated bactericidal reaction, it was also found to be highly specific as well as reproducible. Experiments which studied the effects of antigen type (live or heat-killed), antigen dose, and the route of immunization indicated that the ...

Saito, Kazuko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Experimental salmonellosis. VI. In vitro transfer of cellular immunity of mouse mononuclear phagocytes. J. Bacteriol. 90:629-634. 1965.-A culture medium of the mononuclear phagocytes (monocytes) of mice immunized with live vaccine of Salmonella enteritidis contains the transfer agent (TA) of cellular immunity from immune ...

BACKGROUND Salmonella enterica serovar Pullorum (S. Pullorum) causes Pullorum disease (PD), a severe systemic disease of poultry and results in considerable economic losses in developing countries. In order to develop a safe and immunogenic vaccine, the immunogenicity and protective efficacy of S06004ΔSPI2, a Salmonella pathogenicity island 2 (SPI2) deleted mutant of S. Pullorum was evaluated i...