نتایج جستجو برای: assisted oocyte activation

تعداد نتایج: 568818  

Aghajanpour S, Deemeh MR Ghaedi K Nasr Esfahani MH, Salamian A Tavalaee M

Background: Failed fertilization post ICSI has been mainly attributed to the sperm’s inability to induce oocyte activation. Phospholipase C Zeta (PLCζ) is considered to be a primary factor for the induction of oocyte activation. The aim of this study was to quantitatively assess the expression of PLCζ in globozoospermic or individuals with previous low or failed fertilization in comparison to f...

2016
Shuai Liu Xiao Qun Liu Hai Yan Huang Zhang Quan Gao Xiang Gai Zhang

The successful outcome of intracytoplasmic sperm injection (ICSI) with round-headed spermatozoa (globozoospermia) and ionomycin assisted activation oocytes is reported. A 30-year-old man with 100% globozoospermia underwent ICSI treatment. Real-time Polymerase Chain Reaction (PCR) detection indicates that the patient was DPY19L2 homozygous deletion. Nine metaphase II oocytes were injected with r...

2013
Manoj Kumar Kishlay Kumar Shalu Jain Tarannum Hassan Rima Dada

The integrity of the sperm genome and epigenome are critical for normal embryonic development. The advent of assisted reproductive technology has led to an increased understanding of the role of sperm in fertilization and embryogenesis. During fertilization, the sperm transmits not only nuclear DNA to the oocyte but also activation factor, centrosomes, and a host of messenger RNA and microRNAs....

Journal: :Endocrinology 2016
Federica Franciosi Shila Manandhar Marco Conti

A major challenge in assisted reproductive technology is to develop conditions for in vitro oocyte maturation yielding high-quality eggs. Efforts are underway to assess whether known hormonal and local factors play a role in oocyte developmental competence and to identify the molecular mechanism involved. Here we have tested the hypothesis that FSH improves oocyte developmental competence by re...

2014
Pravin T. Goud Anuradha P. Goud Tohid Najafi Bernard Gonik Michael P. Diamond Ghassan M. Saed Xueji Zhang Husam M. Abu-Soud

Nitric oxide (NO) is reported to play significant a role in oocyte activation and maturation, implantation, and early embryonic development. Previously we have shown that NO forms an important component of the oocyte microenvironment, and functions effectively to delay oocyte aging. Thus, precise information about intra-oocyte NO concentrations [NO] will result in designing more accurate treatm...

2014
Irma Virant-Klun Jeroen Krijgsveld

Oocytes are crucial cells for mammalian reproduction, yet the molecular principles underlying oocyte development are only partially understood. Therefore, contemporary proteomic approaches have been used increasingly to provide new insights into oocyte quality and maturation in various species such as mouse, pig, and cow. Especially, animal studies have helped in elucidating the molecular statu...

2017
Tao Wang Ying-Ying Gao Li Chen Zheng-Wen Nie Wei Cheng Xiaoyan Liu Heide Schatten Xia Zhang Yi-Liang Miao

Oxidative stress is known as a major contributing factor involved in oocyte aging, which negatively affects oocyte quality and development after fertilization. Melatonin is an effective free radical scavenger and its metabolites AFMK and AMK are powerful detoxifiers that eliminate free radicals. In this study, we used porcine oocytes to test the hypothesis that melatonin could scavenge free rad...

Journal: :International journal of andrology 2008
S Grunewald T M Said U Paasch H-J Glander A Agarwal

Human sperm have been documented to display apoptosis-like features such as externalization of phosphatidylserine (EPS), disruption of the transmembrane mitochondrial potential (MMP) and activation of caspases. Our aim was to evaluate possible association between activation of the apoptosis cascade in human sperm and its oocyte penetration capacity using the zona free hamster oocyte penetration...

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