Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry was introduced over 30 years ago, and it now plays a key role in several areas, including the characterization of microorganisms tissue imaging. Lasers are, course, central component MALDI spectrometers, but commercial systems, type is determined by instrument manufacturer, for operator, their only practical consideration sel...

Laser desorption/ionization techniques were firstly developed in the early seventieth years [1] but only in the late eighteenth [2, 3], with the introduction of matrix-assisted laser desorption/ionization, they became an established method for the mass spectrometry of macromolecular compounds. The role of matrix is to absorb UV laser radiation and to give the energy to the analyte, often not ab...

A novel strategy combining peptide fingerprinting of single neurons by matrix-assisted laser desorption ionization mass spectrometry, molecular cloning, peptide chemistry, and electrospray ionization mass spectrometry was used to study the intricate processing pattern of a preprohormone expressed in identified neurons, the neuroendocrine light yellow cells (LYCs) of the gastropod mollusc, Lymna...

T detection and identification of molecules within complex biological matrixes (i.e., plant tissue) requires homogenization, filtration, and liquid extraction of the sample to prepare for analysis using techniques such as gas chromatography/mass spectrometry (GC/MS), liquid chromatography-mass spectrometry (LC-MS), capillary electrophoresis-mass spectrometry (CE-MS), and LC-NMR. Direct analysis...

Characterising the protein signatures in tumours following vascular-targeted therapy will help determine both treatment response and resistance mechanisms. Here, mass spectrometry imaging and MS/MS with and without ion mobility separation have been used for this purpose in a mouse fibrosarcoma model following treatment with the tubulin-binding tumour vascular disrupting agent, combretastatin A-...

Canada. Rapid responses are based on a limited literature search and are not comprehensive, systematic review s. The intent is to provide a list of sources of the best evidence on the topic that CADTH could identify using all reasonable efforts w ithin the time allow ed. Rapid responses should be considered along w ith other types of information and health care considerations. The information i...

We report the case of a total hip arthroplasty infection caused by Ruminococcus gnavus in a 62-year-old man with ulcerative colitis. The bacterium was perfectly identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Laser-induced acoustic desorption (LIAD) has recently been established as a tool for analytical chemistry. It is capable of launching intact, neutral, or low charged molecules into a high vacuum environment. This makes it ideally suited to mass spectrometry. LIAD can be used with fragile biomolecules and very massive compounds alike. Here, we apply LIAD time-of-flight mass spectrometry (TOF-MS)...

We show that ultrafast surface science studies using the laser-assisted photoelectric effect can benefit from longer-wavelength infrared dressing beams. We compare soft-x-ray photoemission from a Pt 111 surface dressed by 1300 and 780 nm light. Using 1300 nm light, the amplitude of the laser-assisted photoelectric signal is enhanced sevenfold compared with 780 nm. This allows lower dressing las...

In this paper, we describe systematic detailed considerations of the feasibility of using various metal nanoparticles for organic-matrix-free surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). In order to avoid the influence of organic molecules on the nanoparticles, stabilizer-free bare nanoparticles of Ag, Au, Cu and Pt were prepared by laser ablation. Although all met...