Highly self-ordered alumina nanopore arrays were fabricated using hard anodization technique in different mixtures of oxalic/phosphoric acid. The phosphoric acid concentration was varied from 0.05 to 0.3 M while the oxalic acid concentration was changed between 0.3 and 0.4 M. The self ordered nanoporous arrays were obtained in anodization voltage changing from 130 to 200 V. The interpore distan...

MicroRNAs (miRNAs) are short, endogenous non-coding RNAs that function as guide molecules to regulate transcription of their target messenger RNAs. Several methods including low-density qPCR arrays are being increasingly used to profile the expression of these molecules in a variety of different biological conditions. Reliable analysis of expression profiles demands removal of technical variati...

Brain functions are strictly dependent on neural connections formed during development and modified during life. The cellular and molecular mechanisms underlying synaptogenesis and plastic changes involved in learning and memory have been analyzed in detail in simple animals such as invertebrates and in circuits of mammalian brains mainly by intracellular recordings of neuronal activity. In the...

DNA microarrays are widely used as end point detectors for gene expression analysis. Several methods have been developed for target labelling to enable quantification but without taking target length into consideration. Here we highlight the importance of choosing the optimum target length that would ensure specificity without compromising sensitivity of the assay. For this, eight plasmids that...

We investigate the problem of designing efficient multiplex PCR for medical applications. We show that the problem is NP-complete by transformation to the Multiple Choice Matching problem and give an efficient approximation algorithm. We developed this algorithm in a computer program that predicts which genomic regions may be simultaneously amplified by PCR. Practical use of the software shows ...

BACKGROUND More than two decades after its discovery, contaminant microbial DNA in PCR reagents continues to impact the sensitivity and integrity of broad-range PCR diagnostic techniques. This is particularly relevant to their use in the setting of human sepsis, where a successful diagnostic on blood samples needs to combine universal bacterial detection with sensitivity to 1-2 genome copies, b...

‎the main purpose of this note is to establish some bounds in poisson approximation for row-wise arrays of independent geometric distributed random variables using the operator method‎. ‎some results related to random sums of independent geometric distributed random variables are also investigated.

Discrete Spherical Harmonic Transforms (SHTs) are commonly defined for equiangular grids on the sphere. However, when global array data exhibit near equidistributed patterns rather than equiangular grids, discrete SHTs require appropriate adaptations for analysis and synthesis. Computational efficiency and reliability impose structural constraints on possible equidistribution characteristics of...