Three urea cycle enzymes, argininosuccinate synthetase, argininosuccinate lyase, and argÃ1⁄4Ã-ase, and two amino acyl-RNA synthetases, the glutamyl and the glutaminyl enzymes, have been studied in the Novikoff hepatoma and Morris Hepatomas 7787, 7793, and 7800. Except for Hepatoma 7793, which had "normal" activities of the 3 urea cycle enzymes, other hepatomas had lower activities than normal l...

*Corresponding Author Rajesh. K. Patel [email protected] The present study investigated the occurrence of an autosomal recessive genetic disease, Bovine Citrullinaemia caused by mutation in Argininosuccinate Synthase (ASS) gene, in Indian Holstein cattle. The Polymerase chain reactionRestriction fragment length polymorphism (PCR-RFLP) analysis was performed on a group of 120 Holstein bulls to...

The role of arginine as an essential amino was evaluated in four children with one of the deficiencies of carbamyl phosphate synthetase, ornithine transcarbamylase, argininosuccinate synthetase, and argininosuccinase. Within 15-68 h after arginine deprivation nitrogen accumulated as ammonium or glutamine or both, but glutamine was quantitatively the largest nitrogen accumulation product. Concom...

Metabolism as well as function of the three amino acids, L-arginine, L-citrulline, and Lornithine, are closely intertwined and related to the homeostasis of nitric oxide at the tissue and cellular levels. For the three compounds data on neural tissue and CSF levels are summarized and localization in brain mainly derived from immunohistochemical experiments is reported. A complex pattern of diff...

Endothelial nitric oxide synthase (eNOS) utilizes L-arginine as its principal substrate, converting it to L-citrulline and nitric oxide (NO). L-citrulline is recycled to L-arginine by two enzymes, argininosuccinate synthase (AS) and argininosuccinate lyase (AL), providing the substrate arginine for eNOS and NO production in endothelial cells. Together, these three enzymes, eNOS, AS and AL, make...

Somatotropin (ST) treatment promotes animal growth and allows for the conservation of amino acids by increasing nitrogen retention and reducing ureagenesis and amino acid oxidation. To determine whether the improvement in amino acid conservation with ST treatment involves regulation of urea cycle enzyme activities in both liver and intestine, growing swine were treated with either ST (150 g kg ...

Three urea cycle enzymes, argininosuccinate synthetase, argininosuccinate lyase, and argÃ1⁄4Ã-ase, and two amino acyl-RNA synthetases, the glutamyl and the glutaminyl enzymes, have been studied in the Novikoff hepatoma and Morris Hepatomas 7787, 7793, and 7800. Except for Hepatoma 7793, which had "normal" activities of the 3 urea cycle enzymes, other hepatomas had lower activities than normal l...

The levels of the activities of argininosuccinate synthetase and argininosuccinate lyase were measured in soybean (glycine max L. var. Mandarin) cell suspension cultures grown in the presence or absence of exogenous arginine. In some experiments, actinomycin D or cycloheximide were also added to the cultures, at critical stages of their growth. The results obtained led to the conclusion that ac...