Despite being known as resistant proteins, peanut allergens (Ara h 1 and Ara h 2) can be digested and cause allergic reactions. Making the allergens more resistant to digestion may aid in non-absorption and excretion of the allergens. Our objectives were to make Ara h 1 and Ara h 2 more resistant to digestion and test them in a model system using trypsin as the digestive enzyme. The resistant a...

The clinical role of Ara h 6 sensitization in peanut allergy is a current matter of debate. We investigated the role of Ara h 6 sensitization patterns in a sample of young adults from different Italian cities. Sera of 33 patients with specific IgE against Ara h 6 were selected. According to clinical symptoms upon peanut ingestion, patients were divided into severe reaction (SR) and mild-toleran...

PURPOSE The purpose of this study was to determine the influence of Maillard reaction (MR, glycation) on biochemical and biological properties of the major peanut allergen Ara h 1. METHODS Three different time/temperature conditions of treatment were applied (37, 60, and 145 °C). The extent of MR was assessed by SDS-PAGE, loss of free amino groups, fluorescence intensity, content of bound sug...

BACKGROUND Modification of native peanut extracts could reduce adverse effects of peanut immunotherapy. OBJECTIVE We sought to compare native and chemically modified crude peanut extract (CPE) and major peanut allergens Ara h 2 and Ara h 6 in a mediator-release assay based on the rat basophilic leukemia (RBL) cell line transfected with human Fcε receptor. METHODS Native Ara h 2/6 was reduce...

BACKGROUND Measurement of IgE antibody to peanut components can aid in the prediction of allergic responses the food. OBJECTIVE To investigate the association between patient demographics (age, location) and allergic sensitization to peanut components across the United States. METHODS Serum samples from 12,155 individuals with peanut extract specific IgE levels of 0.35 kUA/L or higher were ...

Resistance to proteolytic enzymes and heat is thought to be a prerequisite property of food allergens. Allergens from peanut (Arachis hypogaea) are the most frequent cause of fatal food allergic reactions. The allergenic 2S albumin Ara h 2 and the homologous minor allergen Ara h 6 were studied at the molecular level with regard to allergenic potency of native and protease-treated allergen. A hi...

BACKGROUND Peanut allergy is one of the most severe food allergies in children. The diagnostic gold standard is the oral food challenge (OFC). However, OFC has inherent risks and is time consuming. The measurement of specific immunoglobulin E (sIgE) to peanut components in blood detects peanut sensitization, but the decision point predicting allergy is still unclear. The aim of this study was t...

Background Peanut allergy is considered one of the most prominent food allergies, which often results in severe reactions and can be fatal. It can be attributed to one or more of the 12 recognised peanut allergens, the main peanut allergens being Ara h 1, Ara h 2/6 and Ara h 3. Ara h 2/6 are 2S albumins, sensitisation to which is associated with the most severe allergic reactions. They are used...