Activated complement is an important mediator of inflammation. Radioimmunoassay was used to measure levels of C3a, an activated fragment of C3, in aqueous humor. Additionally, immunoelectrophoresis was performed on aqueous humor to detect Factor B and its conversion product, Bb, as well as C3c, a breakdown product of C3. All six samples of normal aqueous humor had no detectable C3a, C3c, or Fac...

By using a highly sensitive and specific radioimmunoassay and the slot-blot technique, transferrin was quantified in fresh samples of aqueous humor from patients with primary open-angle glaucoma (POAG, n = 36) or secondary glaucoma (SG, n = 18). The levels were compared with those in aqueous humor obtained from age-matched patients without glaucoma (n = 33) and in primary and secondary aqueous ...

PURPOSE To investigate the aqueous humor proteome in patients with glaucoma and a control group. METHOD Aqueous humor was obtained from five human donors diagnosed with primary open angle glaucoma (POAG) and five age- and sex-matched controls undergoing cataract surgery. Quantitative proteome analysis of the aqueous humor by hyper reaction monitoring mass spectrometry (HRM-MS) based on SWATH ...

PURPOSE To assess aqueous humor dynamics in mouse eyes. METHODS Aqueous humor dynamics of NIH Swiss White mouse were assessed with an injection and aspiration system, using fine glass microneedles. Intraocular pressure (IOP) was measured by a microneedle connected to a pressure transducer. Episcleral venous pressure (EVP) was measured by gradually lowering intracameral pressure until blood re...

Ocular penetration of 5-fluorocytosine (5FC) was studied in uninfected rabbits after subconjunctival and oral administration. With oral administration, 5FC achieved therapeutic levels in both the vitreous and aqueous humors. The use of a pharmacokinetic model permitted objective comparison of kinetic events within the eye chambers and the serum. The rates of entry and elimination in the vitreou...

EARLY analyses of the proteins of the normal aqueous humour by Franceschetti and Wieland (1928), and later by Kronfeld (1941) and Kronfeld and others (1941), have shown a content of 10 to 20 mg. per cent. Any functional significance this protein may have is unknown. However, the human lens contains 30-40 p' r cent. protein, the fractions of which have been determined by several authors (Cagianu...

Previous work on the vitamin B12 content of the aqueous humour (Phillips, Ainley, van Peborgh, Watson-Williams, and Bottomley, I968) suggests that the levels found in a series of mainly elderly patients (mean: 299 pg./ml.) are of the same order as those found in cerebrospinal fluid (mean: 21 pg./ml.) (Kidd, Gould, and Thomas, I963). As an extension of this study, the response of aqueous vitamin...

Although the lens has been shown to have the capacity to respond to insulin in vitro, little is known concerning the biochemical relationships of insulin to the lens in vivo. Therefore we have measured insulin in the aqueous humor of rabbits by a sensitive radioimmunoassay after paracentesis and feeding. The insulin concentration in aqueous humor was 3% of that in plasma. One hour after paracen...

purpose: to investigate aqueous humor nitric oxide (no) levels in patients with branch retinal vein occlusion (brvo) and central retinal vein occlusion (crvo) and to compare these with age-matched controls. methods: eight consecutive patients with brvo and 16 patients with crvo were included in this study. aqueous humor specimens were obtained within 21 days of diagnosis. samples of aqueous hum...

It has recently been shown that aqueous humor is able to obstruct flow through Nuclepore polycarbonate filters having flow dimensions similar to those found in the juxtacanalicular tissue (JCT). We undertook studies designed to identify the component(s) of aqueous humor responsible for this obstruction and to determine the mechanism of blockage. We conclude that aqueous humor contains two compo...