In order to increase the yield of haploid cucumber plants obtained from in vitro culture of unpollinated ovules, anther and microspore, we have studied several factors: the nature and concentration of plant growth regulators, the genotypes, the flower bud lengths, and the stages of microspore development. Among the different growth regulators tested, thidiazuron (TDZ) was the best for gynogenes...

Different experimental conditions have been compared to achieve a high efficiency in embryogenic calli initiation from 'Macabeo' and 'Tempranillo' anthers. Specifically, two stages of anther development were tested (corresponding to tetrad cells or uninucleate pollen), and direct culture of anthers was compared to culture after a cold treatment of inflorescences (4 °C during 48 h). In addition,...

Legumes are an alternative to animal foods due their high protein content. Like other legumes, Vicia faba (faba bean) has However, bean breeding with classical methods is challenging inbreeding depression, self-incompatibility, and abortive embryo formation. The inadequacy of the problems in fertilization biology plant limits production new varieties. Therefore, importance using vitro haploidiz...

The effect of three concentrations of silver nitrate on anther culture embryo production was studied in 27 morphotypes of Brassica oleracea. Embryo yields were significantly increased in the majority of the morphotypes by the addition of silver nitrate to the media. Embryo yields were not increased in only a few of the almost nonembryogenic accessions. The magnitude of the response to silver ni...

sugar type and growth regulators are essential fundamental components of tissue culture media. in this study, different concentrations of some carbohydrate and growth regulators during anther culture and producing somatic embryo of datura stramonium l. was investigated in ms medium. the ms medium supplemented with 0.5 mgl-1 kinetin and 2 mgl-1 2,4-d, and also the medium supplemented with 0.25 m...

Double haploid technique is not routinely used in legume breeding programs, though recent publications report haploid plants via anther culture in chickpea (Cicer arietinum L.). The focus of this study was to develop an efficient and reproducible protocol for the production of double haploids with the application of multiple stress pre-treatments such as centrifugation and osmotic shock for gen...